Ring Finger Protein 216 (RNF216) antibody

Details for Product No. ABIN1003372
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms RGD1565135, TRIAD3, RNF216, zin, u7i1, triad3, ubce7ip1, MGC147026, 2810055g22rik, 2810055G22Rik, AI647468, AU019462, C86502, F830018F18Rik, UIP83, Ubce7ip1, CAHH, U7I1, UBCE7IP1, ZIN
Reactivity
Mouse (Murine)
(37), (33), (12), (12)
Host
Rabbit
(49), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(38), (16), (16), (12), (10), (4), (1)
Pubmed 4 references available
Quantity 100 µg
Shipping to United States (Change)
Availability Will be delivered in 6 to 7 Business Days
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN1003372
514.01 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Rabbit polyclol TRIAD3A antibody was raised against a peptide corresponding to 15 amino acids near the amino-terminus of mouse TRIAD3A (GenBank accession no. AAP47174).
Isotype IgG
Purification Ion exchange chromatography purified.
Alternative Name TRIAD3A (TLR Ubiquitinating Enzyme)
Background Activation of NF-kappaB as a result of Toll-like receptor (TLR) and IL-1 receptor signaling is a major component of innate immune responses. Signals from these receptors are relayed by a number of adapter molecules such as TRIF, TIRAP, and MyD88. Several regulatory mechanisms exist to control TLR signal transduction, including the inhibition of TLR expression and signaling by molecules such as ST2 and SIGIRR. Another mechanism is by the ubi-quitinization of selected TLRs by TRIAD3A, an E3 ubiquitin-protein ligase. TRIAD3A is a RING finger protein that can bind to TLR4 and TLR9, and to a lesser extent TLR3 and TLR5, catalyzing the ubiquitization of these molecules. Overexpression of TRIAD3A promoted the nearly complete degradation of TLR4 and TLR9, this reduction was reflected in the decreased signal-specific activation by ligands specific for these TLRs. Conversely, depletion of TRIAD3A resulted in enhanced TLR activation.
Application Notes TRIAD3A antibody can be used for detection of TRIAD3A by Western blot at 0.5 to 1 µg/ml. (Optimal dilution should be determined by user.) Antibody can also be used for immunohistochemistry and ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Product cited in: Sweet, Leung, Kang et al.: "A novel pathway regulating lipopolysaccharide-induced shock by ST2/T1 via inhibition of Toll-like receptor 4 expression." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 11, pp. 6633-9, 2001 (PubMed).

Takeda, Kaisho, Akira: "Toll-like receptors." in: Annual review of immunology, Vol. 21, pp. 335-76, 2003 (PubMed).

Wald, Qin, Zhao et al.: "SIGIRR, a negative regulator of Toll-like receptor-interleukin 1 receptor signaling." in: Nature immunology, Vol. 4, Issue 9, pp. 920-7, 2003 (PubMed).

Vogel, Fitzgerald, Fenton: "TLRs: differential adapter utilization by toll-like receptors mediates TLR-specific patterns of gene expression." in: Molecular interventions, Vol. 3, Issue 8, pp. 466-77, 2004 (PubMed).

Validation Images
Did you look for something else?
back to top