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TRIM25 antibody (Tripartite Motif Containing 25) (C-Term)

Details for Product anti-TRIM25 Antibody No. ABIN1003374, Supplier: Log in to see
Antigen
  • AA960166
  • AL022677
  • Efp
  • EFP
  • MGC130751
  • RNF147
  • Z147
  • Zfp147
  • zgc:55773
  • Znf147
  • ZNF147
Epitope
C-Term
18
15
14
14
12
12
11
5
5
4
3
3
2
2
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
123
31
18
1
1
1
1
1
1
Host
Rabbit
110
13
Clonality
Polyclonal
Conjugate
This TRIM25 antibody is un-conjugated
4
4
4
2
2
2
2
2
2
2
2
2
2
2
2
Application
Immunocytochemistry (ICC), ELISA, Western Blotting (WB)
92
51
24
23
18
17
16
5
3
2
2
Supplier
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Immunogen TRIM25 antibody was raised in rabbits against a 19 amino acid peptide from near the carboxy terminus of human TRIM25.
Blocking Peptide Blocking peptide for this product available: ABIN1003906
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Alternative Name TRIM25 (TRIM25 Antibody Abstract)
Background TRIM25, also known as estrogen responsive finger protein (EFP) is a member of the RING finger-B box-coiled coil family and is a downstream target of estrogen receptor a. Mice lacking this gene displayed an underdeveloped uterus and reduced estrogen responsiveness demonstrating that this protein is essential for estrogen dependent proliferation. TRIM25 expression is increased in breast carcinoma where it promotes the growth of breast tumor by functioning as an E3 ubiquitin ligase that targets the negative cell cycle checkpoint 14-3-3s. Recent reports have indicated that TRIM25 E3 ubiquitin ligase is essential for the retinoic-acid-inducible gene 1 (RIG-1) signaling pathway to elicit host antiviral innate immunity.
Application Notes TRIM25 antibody can be used for detection of TRIM25 by Western blot at 1 – 2 µg/ml. (Optimal dilution should be determined by user). Antibody can also be used for immunocytochemistry and ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Product cited in: Urano, Saito, Tsukui, Fujita, Hosoi, Muramatsu, Ouchi, Inoue: "Efp targets 14-3-3 sigma for proteolysis and promotes breast tumour growth." in: Nature, Vol. 417, Issue 6891, pp. 871-5, 2002

Thomson, Ali, Pickles, Taylor, Pace, Lymboura, Shousha, Coombes: "Analysis of estrogen-responsive finger protein expression in benign and malignant human breast." in: International journal of cancer. Journal international du cancer, Vol. 91, Issue 2, pp. 152-8, 2001

Orimo, Inoue, Minowa, Tominaga, Tomioka, Sato, Kuno, Hiroi, Shimizu, Suzuki, Noda, Muramatsu: "Underdeveloped uterus and reduced estrogen responsiveness in mice with disruption of the estrogen-responsive finger protein gene, which is a direct target of estrogen receptor alpha." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 21, pp. 12027-32, 1999

Inoue, Orimo, Hosoi, Kondo, Toyoshima, Kondo, Ikegami, Ouchi, Orimo, Muramatsu: "Genomic binding-site cloning reveals an estrogen-responsive gene that encodes a RING finger protein." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 23, pp. 11117-21, 1994