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Ube2t antibody (Ubiquitin-Conjugating Enzyme E2T (Putative))

Details for Product anti-Ube2t Antibody No. ABIN1003408, Supplier: Log in to see
  • 2700084L22Rik
  • C80607
  • HR6B
  • hspc150
  • PIG50
  • RGD1310816
  • UBE2T
  • wu:fj86h11
  • zgc:153687
Human, Mouse (Murine), Rat (Rattus)
This Ube2t antibody is un-conjugated
ELISA, Western Blotting (WB)
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Immunogen Rabbit polyclol UEV1A antibody was raised against a peptide corresponding to 15 amino acids near the C-terminus of human UEV1A (GenBank accession no. NP_954595).
Blocking Peptide Blocking peptide for this product available: ABIN1004496
Isotype IgG
Purification Ion exchange chromatography purified.
Alternative Name Ubiquitin Conjugating Enzyme (Ube2t Antibody Abstract)
Background Ubiquitin-conjugating enzyme E2 (UEV1) was initially discovered as a protein similar in sequence and structure to the E2 ubiquitin-conjugating enzymes but lacking their enzymatic activity. There are at least two variants and multiple isoforms of UEV1. In particular, UEV1A (Ubiquitin-conjugating enzyme E2 variant 1 isoform A) has recently been shown to be an important component of the Toll-like receptor and IL-1R signaling pathway. Signals from these pathways are relayed by a number of downstream molecules such as MyD88 and tumor necrosis factor receptor associated factor (TRAF6), ultimately activating various kinases and transcription factors. UEV1A is part of a dimeric ubiquitin-conjugating enzyme complex also containing Ubc13 (ubiquitin-conjugating enzyme 13) that together with TRAF6 activates TAK1, a member of the mitogen-activated protein kinase kinase kinase family. The Ubc13-UEV1A complex also mediates the Lys-63 ubiquitination of TRAF-6, and this ubiquitination is essential for TAK1 activation.
Application Notes UEV1A antibody can be used for detection of UEV1A by Western blot at 1 to 2 µg/ml. (Optimal dilution should be determined by user.) Anti-UEV1A may also recognize other isoforms of UEV1A. Antibody can also be used for ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Product cited in: Akira, Takeda: "Toll-like receptor signalling." in: Nature reviews. Immunology, Vol. 4, Issue 7, pp. 499-511, 2004 (PubMed).

Background publications Vogel, Fitzgerald, Fenton: "TLRs: differential adapter utilization by toll-like receptors mediates TLR-specific patterns of gene expression." in: Molecular interventions, Vol. 3, Issue 8, pp. 466-77, 2004 (PubMed).

Deng, Wang, Spencer, Yang, Braun, You, Slaughter, Pickart, Chen: "Activation of the IkappaB kinase complex by TRAF6 requires a dimeric ubiquitin-conjugating enzyme complex and a unique polyubiquitin chain." in: Cell, Vol. 103, Issue 2, pp. 351-61, 2000 (PubMed).

Sancho, Vilá, Sánchez-Pulido, Lozano, Paciucci, Nadal, Fox, Harvey, Bercovich, Loukili, Ciechanover, Lin, Sanz, Estivill, Valencia, Thomson: "Role of UEV-1, an inactive variant of the E2 ubiquitin-conjugating enzymes, in in vitro differentiation and cell cycle behavior of HT-29-M6 intestinal mucosecretory cells." in: Molecular and cellular biology, Vol. 18, Issue 1, pp. 576-89, 1998 (PubMed).