VPS53 (Vacuolar Protein Sorting 53, HCCS1) (C-Term) antibody

Details for Product No. ABIN1003472, Supplier: Log in to see
Human, Mouse (Murine), Rat (Rattus)
Immunocytochemistry (ICC), ELISA, Western Blotting (WB)
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Immunogen VPS53 antibody was raised in rabbits against an 18 amino acid peptide from near the carboxy terminus of human VPS53.
Blocking Peptide Blocking peptide for this product available: ABIN1003913
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Background The sorting of acid hydrolases to lysosomes rely on mannose 6-phosphate receptors that cycle between the trans-Golgi network (TGN) and endosomes. The maintenance of this cycle requires the function of the mammalian Golgi-associated retrograde protein (GARP) complex which is composed of three subunits: VPS52, VPS53, and VPS54. Depletion of any of these three proteins, such as by RNAi, impairs the retrograde transport of multiple TGN proteins. VPS53 was identified as an HIV dependency factor (HDF) and plays a role in viral entry to the cell, suggesting that VPS53 may be an important drug target in HIV treatment. At least five isoforms of VPS53 are known to exist.
Application Notes VPS53 antibody can be used for detection of VPS53 by Western blot at 0.5 – 1 µg/ml. (Optimal dilution should be determined by user). Antibody can also be used for immunocytochemistry and ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Product cited in: Pérez-Victoria, Mardones, Bonifacino: "Requirement of the human GARP complex for mannose 6-phosphate-receptor-dependent sorting of cathepsin D to lysosomes." in: Molecular biology of the cell, Vol. 19, Issue 6, pp. 2350-62, 2008 (PubMed).

Brass, Dykxhoorn, Benita, Yan, Engelman, Xavier, Lieberman, Elledge: "Identification of host proteins required for HIV infection through a functional genomic screen." in: Science (New York, N.Y.), Vol. 319, Issue 5865, pp. 921-6, 2008 (PubMed).

Liewen, Meinhold-Heerlein, Oliveira, Schwarzenbacher, Luo, Wadle, Jung, Pfreundschuh, Stenner-Liewen: "Characterization of the human GARP (Golgi associated retrograde protein) complex." in: Experimental cell research, Vol. 306, Issue 1, pp. 24-34, 2005 (PubMed).

Pfeffer: "Targeting of proteins to the lysosome." in: Current topics in microbiology and immunology, Vol. 170, pp. 43-65, 1992 (PubMed).