You are viewing an incomplete version of our website. Please click to reload the website as full version.

XBP1 antibody (X-Box Binding Protein 1) (C-Term)

Details for Product anti-XBP1 Antibody No. ABIN1003489, Supplier: Log in to see
Antigen
  • treb5
  • xXBP1
  • xbp2
  • XBP1P1
  • XBPP1
  • xbp1
  • TREB5
  • XBP-1
  • XBP2
  • D11Ertd39e
  • TREB-5
  • HTF
  • cb918
  • id:ibd1195
  • trebf
  • wu:fb02h05
  • wu:fb63f10
  • xbp1a
  • xbp1b
  • X-box binding protein 1
  • uncharacterized LOC100719734
  • X box-binding protein active isoform
  • xbp1
  • XBP1
  • LOC100719734
  • LOC100037692
  • Xbp1
Epitope
C-Term
32
32
18
18
15
13
13
13
12
9
9
9
7
6
6
4
3
3
2
2
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
205
104
61
6
5
5
3
2
2
1
1
1
1
Host
Rabbit
131
67
20
5
Clonality
Polyclonal
Conjugate
This XBP1 antibody is un-conjugated
8
7
6
6
6
6
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), ELISA, Western Blotting (WB)
180
105
57
44
40
27
27
13
8
6
3
2
1
1
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Immunogen Rabbit polyclol XBP-1 antibody was raised against a 17 amino acid peptide from near the carboxy terminus of human XBP-1 (Genbank accession No. P17861).
Blocking Peptide Blocking peptide for this product available: ABIN2862240
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Alternative Name X-box Binding Protein 1 (XBP1 Antibody Abstract)
Background X box binding protein 1 (XBP-1) is a key protein in the mammalian unfolded protein response (UPR) that protects the cell against the stress of malfolded proteins in the endoplasmic reticulum (ER). Upon sensing unfolded proteins, an ER transmembrane endonuclease and kinase termed IRE1p is activated and excises an intron from XBP-1 mRNA. The spliced XBP-1 mRNA results in a 371 amino acid protein (XBP-1s) which is then translocated to the nucleus where it binds to the regulatory elements of downstream genes. Together with other UPR transcription factors such as ATF6, XBP-1 stimulates the production of ER stress proteins including the ER resident protein chaperones glucose regulated protein (GRP) 78 and GRP94.
Application Notes XBP-1 antibody can be used for the detection of XBP-1 by Western blot at 0.5 – 1 myg/ml. (Optimal dilution should be determined by user.) PC-3 cell lysate can be used as positive control. XBP-1 antibody will recognize only the nonspliced isoform of XBP-1. Antibody can also be used for immunocytochemistry and ELISA and might be suited for other applications not tested so far. XBP-1 antibody is human, mouse and rat reactive.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Background publications Yoshida, Matsui, Yamamoto, Okada, Mori: "XBP1 mRNA is induced by ATF6 and spliced by IRE1 in response to ER stress to produce a highly active transcription factor." in: Cell, Vol. 107, Issue 7, pp. 881-91, 2002 (PubMed).

Calfon, Zeng, Urano, Till, Hubbard, Harding, Clark, Ron: "IRE1 couples endoplasmic reticulum load to secretory capacity by processing the XBP-1 mRNA." in: Nature, Vol. 415, Issue 6867, pp. 92-6, 2002 (PubMed).

Haze, Yoshida, Yanagi, Yura, Mori: "Mammalian transcription factor ATF6 is synthesized as a transmembrane protein and activated by proteolysis in response to endoplasmic reticulum stress." in: Molecular biology of the cell, Vol. 10, Issue 11, pp. 3787-99, 1999 (PubMed).

Little, Ramakrishnan, Roy, Gazit, Lee: "The glucose-regulated proteins (GRP78 and GRP94): functions, gene regulation, and applications." in: Critical reviews in eukaryotic gene expression, Vol. 4, Issue 1, pp. 1-18, 1995 (PubMed).