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XBP1 antibody (X-Box Binding Protein 1) (N-Term)

Details for Product anti-XBP1 Antibody No. ABIN1003490, Supplier: Log in to see
Antigen
  • treb5
  • xXBP1
  • xbp2
  • XBP1P1
  • XBPP1
  • xbp1
  • TREB5
  • XBP-1
  • XBP2
  • D11Ertd39e
  • TREB-5
  • HTF
  • cb918
  • id:ibd1195
  • trebf
  • wu:fb02h05
  • wu:fb63f10
  • xbp1a
  • xbp1b
Epitope
N-Term
37
27
18
15
13
13
12
12
9
6
5
4
3
3
2
2
1
1
Reactivity
Human, Mouse (Murine)
167
74
49
4
3
3
1
1
1
1
1
1
Host
Rabbit
111
49
19
5
Clonality
Polyclonal
Conjugate
This XBP1 antibody is un-conjugated
6
6
5
5
5
4
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
162
92
38
28
24
18
11
10
9
7
3
2
1
1
1
Supplier
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Immunogen XBP-1 antibody was raised against a 18 amino acid peptide from near the amino terminus of human XBP-1.
Blocking Peptide Blocking peptide for this product available: ABIN2862241
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Alternative Name X-box Binding Protein 1 (XBP1 Antibody Abstract)
Background X box binding protein 1 (XBP-1) is a key protein in the mammalian unfolded protein response (UPR) that protects the cell against the stress of malfolded proteins in the endoplasmic reticulum (ER). Upon sensing unfolded proteins, an ER transmembrane endonuclease and kinase termed IRE1p is activated and excises an intron from XBP-1 mRNA. The spliced XBP-1 mRNA results in a 371 amino acid protein (XBP-1s) which is then translocated to the nucleus where it binds to the regulatory elements of downstream genes. Together with other UPR transcription factors such as ATF6, XBP-1 stimulates the production of ER stress proteins including the ER resident protein chaperones glucose regulated protein (GRP) 78 and GRP94.
Application Notes XBP-1 antibody can be used for the detection of XBP-1 by Western blot at 1 – 2 µg/ml. (Optimal dilution should be determined by user.) Antibody can also be used for immunohistochemistry and ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Background publications Calfon, Zeng, Urano et al.: "IRE1 couples endoplasmic reticulum load to secretory capacity by processing the XBP-1 mRNA." in: Nature, Vol. 415, Issue 6867, pp. 92-6, 2002 (PubMed).

Yoshida, Matsui, Yamamoto et al.: "XBP1 mRNA is induced by ATF6 and spliced by IRE1 in response to ER stress to produce a highly active transcription factor." in: Cell, Vol. 107, Issue 7, pp. 881-91, 2002 (PubMed).

Haze, Yoshida, Yanagi et al.: "Mammalian transcription factor ATF6 is synthesized as a transmembrane protein and activated by proteolysis in response to endoplasmic reticulum stress." in: Molecular biology of the cell, Vol. 10, Issue 11, pp. 3787-99, 1999 (PubMed).

Little, Ramakrishnan, Roy et al.: "The glucose-regulated proteins (GRP78 and GRP94): functions, gene regulation, and applications." in: Critical reviews in eukaryotic gene expression, Vol. 4, Issue 1, pp. 1-18, 1995 (PubMed).