X-Box Binding Protein 1 (XBP1) (N-Term) antibody

Details for Product No. ABIN1003490
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Antigen
Synonyms TREB5, XBP-1, XBP2, TREB-5, D11Ertd39e, cb918, treb5, trebf, xbp1a, xbp1b, id:ibd1195, wu:fb02h05, wu:fb63f10, HTF, xXBP1, XBPP1, xbp2, XBP1P1, xbp1
Epitope
N-Term
(19), (15), (7), (6), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(115), (58), (45), (15), (15), (13), (4), (1), (1)
Host
Rabbit
(67), (45), (8), (4)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(108), (65), (37), (32), (11), (10), (8), (6), (5), (3), (2), (2), (2), (1)
Pubmed 4 references available
Catalog no. ABIN1003490
Quantity 100 µg
Price
514.01 $   Plus shipping costs $45.00
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Immunogen XBP-1 antibody was raised against a 18 amino acid peptide from near the amino terminus of human XBP-1.
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Alternative Name X-box Binding Protein 1
Background X box binding protein 1 (XBP-1) is a key protein in the mammalian unfolded protein response (UPR) that protects the cell against the stress of malfolded proteins in the endoplasmic reticulum (ER). Upon sensing unfolded proteins, an ER transmembrane endonuclease and kinase termed IRE1p is activated and excises an intron from XBP-1 mRNA. The spliced XBP-1 mRNA results in a 371 amino acid protein (XBP-1s) which is then translocated to the nucleus where it binds to the regulatory elements of downstream genes. Together with other UPR transcription factors such as ATF6, XBP-1 stimulates the production of ER stress proteins including the ER resident protein chaperones glucose regulated protein (GRP) 78 and GRP94.
Application Notes XBP-1 antibody can be used for the detection of XBP-1 by Western blot at 1 – 2 µg/ml. (Optimal dilution should be determined by user.) Antibody can also be used for immunohistochemistry and ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Handling Advice Antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Product cited in: Little, Ramakrishnan, Roy et al.: "The glucose-regulated proteins (GRP78 and GRP94): functions, gene regulation, and applications." in: Critical reviews in eukaryotic gene expression, Vol. 4, Issue 1, pp. 1-18, 1995 (PubMed).

Haze, Yoshida, Yanagi et al.: "Mammalian transcription factor ATF6 is synthesized as a transmembrane protein and activated by proteolysis in response to endoplasmic reticulum stress." in: Molecular biology of the cell, Vol. 10, Issue 11, pp. 3787-99, 1999 (PubMed).

Yoshida, Matsui, Yamamoto et al.: "XBP1 mRNA is induced by ATF6 and spliced by IRE1 in response to ER stress to produce a highly active transcription factor." in: Cell, Vol. 107, Issue 7, pp. 881-91, 2002 (PubMed).

Calfon, Zeng, Urano et al.: "IRE1 couples endoplasmic reticulum load to secretory capacity by processing the XBP-1 mRNA." in: Nature, Vol. 415, Issue 6867, pp. 92-6, 2002 (PubMed).

Hosts (67), (45), (8), (4)
Reactivities (115), (58), (45), (15), (15), (13), (4), (1), (1)
Applications (108), (65), (37), (32), (11), (10), (8), (6), (5), (3), (2), (2), (2), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (19), (15), (7), (6), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1)
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