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Details for Product No. ABIN1003493

XIAP Associated Factor 1 (Inhibitor of XIAP) (C-Term) antibody

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Antigen
Epitope
»Alternatives C-Term
Reactivity
»Alternatives Human, Mouse (Murine)
Host
»Alternatives Rabbit
Clonality Polyclonal
Application
»Alternatives ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
Pubmed 4 references available
Catalog no. ABIN1003493
Quantity 100 µg
Price
514.01 $   Plus shipping costs $45.00
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Availability Will be delivered in 6 to 7 Business Days
Immunogen Rabbit polyclol XAF-1 antibody was raised against a synthetic peptide corresponding to amino acids at the C-terminus of human XAF-1 (Genbank accession CAA68030).
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Background XAF-1 binds to XIAP, an inhibitor of caspases-3, -7, and -9, and triggers its relocation from the cytosol to the nucleus. Overexpression of XAF-1 results in the neutralization of XIAP's ability to inhibit cell death. XAF-1 is normally expressed in all adult and fetal tissues but was found to be present in very low levels in a variety of cancer cell lines. In contrast, XIAP levels have been shown to be high in a majority of cell lines. Low XAF-1 and high basal expression of XIAP may therefore play a critical role in maintaining survival of cancer cell lines. Both IFN-alpha2 and IFN-beta can induce XAF-1 mRNA in all cells examined but induction of XAF-1 protein (as observed by immunoblot analysis) was seen only in cell lines sensitive to the apoptotic effects of IFNs.
Application Notes XAF-1 antibody can be used for the detection of XAF-1 by Western blot at 0.5 to 2 µg/ml. (Optimal dilution should be determined by user.) A 32 kDa band can be detected. Antibody can also be used for immunohistochemistry and ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Handling Advice Antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Product cited in: Deveraux, Takahashi, Salvesen et al.: "X-linked IAP is a direct inhibitor of cell-death proteases." in: Nature, Vol. 388, Issue 6639, pp. 300-4, 1997 (PubMed).

Fong, Liston, Rajcan-Separovic et al.: "Expression and genetic analysis of XIAP-associated factor 1 (XAF1) in cancer cell lines." in: Genomics, Vol. 70, Issue 1, pp. 113-22, 2001 (PubMed).

Liston, Fong, Kelly et al.: "Identification of XAF1 as an antagonist of XIAP anti-Caspase activity." in: Nature cell biology, Vol. 3, Issue 2, pp. 128-33, 2001 (PubMed).

Leaman, Chawla-Sarkar, Vyas et al.: "Identification of X-linked inhibitor of apoptosis-associated factor-1 as an interferon-stimulated gene that augments TRAIL Apo2L-induced apoptosis." in: The Journal of biological chemistry, Vol. 277, Issue 32, pp. 28504-11, 2002 (PubMed).

Alternatives for antigen "XIAP Associated Factor 1 (Inhibitor of XIAP)", type "Antibodies"
Hosts (1)
Reactivities (1)
Applications (1), (1)
Epitopes (1)
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