HIF3A antibody (AA 581-592)

Catalog No. ABIN100475

Product Details anti-HIF3A Antibody

Target: HIF3A (Hypoxia Inducible Factor 3, alpha Subunit (HIF3A))

Binding Specificity: AA 581-592

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HIF3A antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Characteristics: Concentration Definition: by UV absorbance at 280 nm

Sterility: Sterile filtered

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to aa 581-592 of mouse Hif3 a (Hypoxia Inducible Factor).

Isotype: IgG

Application Details

Application Notes: This affinity-purified antibody has been tested for use in ELISA and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 72 kDa in size corresponding to Hif3 a protein by western blotting in the appropriate cell lysate or extract. This antibody is expected to cross-react with mouse and rat Hif3 a due to sequence homology.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.2 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

References for anti-HIF3A antibody (ABIN100475)

Takeda, Ho, Takeda, Duan, Nagy, Fong: "Placental but not heart defects are associated with elevated hypoxia-inducible factor alpha levels in mice lacking prolyl hydroxylase domain protein 2." in: Molecular and cellular biology, Vol. 26, Issue 22, pp. 8336-46, (2006) (PubMed).

Target Details for HIF3A

Target: HIF3A (Hypoxia Inducible Factor 3, alpha Subunit (HIF3A))

Alternative Name: HIF3 alpha (HIF3A Products)

Synonyms: HIF-3A antibody, IPAS antibody, MOP7 antibody, PASD7 antibody, bHLHe17 antibody, Ipas antibody, NEPAS antibody, Mop7 antibody, HIF3A antibody, hypoxia inducible factor 3 alpha subunit antibody, hypoxia inducible factor 3, alpha subunit antibody, HIF3A antibody, Hif3a antibody

Background: One of the most important factors in the cellular response to hypoxia is hypoxia-inducible factor (HIF), which transcriptionally activates genes encoding proteins that mediate adaptive responses to reduced oxygen availability. HIF is a heterodimer consisting of one of three PRIVATE ''TYPE=PICT;ALT={alpha}''subunits (HIF1-aPRIVATE ''TYPE=PICT;ALT={alpha}'', HIF2-a,PRIVATE ''TYPE=PICT;ALT={alpha}'' or HIF3-a)PRIVATE ''TYPE=PICT;ALT={alpha}'' bound to the aryl hydrocarbon receptor nuclear translocator (ARNT) that is also known as HIF1-b. HIF-aPRIVATE ''TYPE=PICT;ALT={alpha}'' is a member of the basic helix-loop-helix (bHLH) superfamily, in which the HLH domain mediates subunit dimerization while the basic domain binds to DNA. HIF target genes play critical roles in metabolism, angiogenesis, cell proliferation, and cell survival; in fact, HIF3-a may be a marker for tumor growth and angiogenesis . Examples of HIF target genes include VEGF, glucose transporter 1 (GLUT1), and EPO. HIF binds to the hypoxia-responsive
Synonyms: HIF3 alpha antibody, HIF3A antibody, Hypoxia Inducible Factor 3 alpha antibody, Hypoxia inducible factor 3 alpha subunit antibody, Hypoxia inducible factor three alpha antibody, Inhibitory PAS domain protein antibody, IPAS antibody

Gene ID: 53417, 170295859

UniProt: Q0VBL6

Pathways: Warburg Effect