Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase epsilon (IKBKE) (pT501) antibody

Antigen: Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase epsilon (IKBKE)

Synonyms: IKKE, IKKI, IKK-i, KIAA0151, MGC125294, MGC125295, MGC125297, zgc:100844, IKBKE, MGC137373, MGC130907

Epitope: pT501

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Cow (Bovine), Mouse (Murine), Rat (Rattus)

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN100676

Additional Information

Alternative name: IKK epsilon phospho specific pT501

Immunogen: IKKE phospho peptide corresponding to a region of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH). Sequence information: R-S-R-L-R-pT-L-A-E-V-L (aa 496-506)

Format: Liquid (sterile filtered)

Description: Nuclear Factor kappa B (NF- kappaB) is a ubiquitous transcription factor and an essential mediator of gene expression during the activation of immune and inflammatory responses. NF- kappaB mediates the expression of a great variety of genes in response to extracellular stimuli. NF- kappaB is associated with IkappaB proteins in the cytoplasm of the cell, which inhibit NF- kappaB activity. IkappaB proteins are phosphorylated by an IkappaB kinase complex consisting of at least three proteins, IKKalpha, IKKbeta, and IKK gamma. Isolated from a cDNA library of LPS-stimulated mouse macrophage cells, a novel molecule in the IKK complex has been recently identified and designated IKKi and/or IKK ε. IKKε is required for the activation of NF-kappaB by mitogens and T cell receptors but not by TNF alpha or IL-1. LPS increases the IKKε mRNA level in mouse macrophage cell lines. This protein has significant sequence homology with kinase domains of IKK alpha and IKKbeta. Overexpression of wild type IKKε in cells phosphorylates Ser32 and Ser36 of I kappaBalpha.

Specificity: This product was affinity purified from monospecific antiserum by immunoaffinity purification against the phosphopeptide and cross adsorption against the non-phosphorylated form of the peptide followed by non-adsorption against a non-specific peptide backbone to further reduce non-specific reactivity. This phospho specific polyclonal antibody is specific for phosphorylated pT501 human IKKE. Reactivity with non-phosphorylated IKKE is minimal. Cross reactivity with pT501 phosphorylated IKKE from mouse, rat or other species has not been determined.

Application Details

Application Notes: Recommended Dilutions: For immunoblotting a 1:1,000 dilution is recommended. An 85 kDa band corresponding to human IKKE is detected. HeLa cells or TNF inducible KBM-5 cells can be used as a positive control. For ELISA a 1:5,000 to 1:10,000 dilution is recommended. Researchers should determine optimal titers for other applications. Note: This phospho specific polyclonal antibody reacts with phosphorylated human IKKE at pT501 and shows minimal reactivity by western blot with non-phosphorylated IKKE and minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation.

Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.1% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Storage: Store vial at -20° C prior to opening. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening.

Research Area: Transcription Factors

Restrictions: For Research Use only