Goat anti-Monkey IgA (alpha chain) Antibody

Antigen: IgA (alpha chain)

Protein Type: Native

Clonality: Polyclonal

Host: Goat

Reactivity: Monkey

Conjugate: Un-conjugated

Catalog no.: ABIN100737

Additional Information

Alternative name: IgA

Immunogen: Anti-Monkey IgA (alpha chain) was produced by repeated immunization with monkey IgA alpha heavy chain in goat.

Format: Liquid

Isotype: IgA

Description: Anti-Monkey IgA (alpha chain) antibody generated in goat detects specifically monkey IgA (alpha chain). This secondary antibody anti-Monkey is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.

Characteristics: Concentration Definition: by UV absorbance at 280 nm

Application Details

Application Notes: Anti-Monkey IgA (alpha chain) is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.

Concentration: 1.0 mg/mL

Purification: Sterile filtered

Buffer: 0.1 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0

Preservative: 0.01% (w/v) Sodium azide

Storage: Expiration date is one (1) year from date of opening. Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

Research Area: Immunology, Secondary Antibodies

Restrictions: For Research Use only

Images

Western Blot of Goat anti-Monkey IgA (alpha chain) antibody. Lane 1: Human IgA. Lane 2: None. Load: 100 ng per lane. Primary antibody: Monkey IgA (alpha chain) antibody at 1:1000 for overnight at 4°C. Secondary antibody: DyLight™ 800 goat secondary antibody at 1:20,000 for 30 min at RT. Block: MB-070 30 min at RT. Predicted/Observed size: 60 kDa, 60 kDa for Monkey IgA. Other Band(s): None.

Publications

Permar, Wilks, Ehlinger et al.: "Limited contribution of mucosal IgA to SIV-specific neutralizing antibody response and virus Envelope evolution in breast milk of SIV-infected, lactating rhesus monkeys." in: Journal of virology, 2010 (PubMed).

Product: Klatt, Vinton, Lynch et al.: "SIV infection of rhesus macaques results in dysfunctional T- and B-cell responses to neo and recall Leishmania major vaccination." in: Blood, 2011 (PubMed). (1:10000).