Goat anti-Rat (Rattus) Immunoglobulin A, Immunoglobulin G, Immunoglobulin M (IgA, IgG, IgM) Antibody (Rhodamine)

Antigen: Immunoglobulin A, Immunoglobulin G, Immunoglobulin M (IgA, IgG, IgM)

Clonality: Polyclonal

Host: Goat

Reactivity: Rat (Rattus)

Conjugate: Rhodamine

Catalog no.: ABIN100823

Additional Information

Alternative name: IgG IgA IgM

Immunogen: Rat IgG, IgA and IgM whole molecules

Format: Lyophilized

Reconstitution: Restore with deionized water (or equivalent)

Isotype: IgG

Description: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Characteristics: Concentration Definition: by UV absorbance at 280 nm

Specificity: IgG IgA IgM

Application Details

Application Notes: FLISA Dilution: 1:10,000 - 1:50,000
IF Microscopy Dilution: 1:1,000 - 1:5,000

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Storage: Expiration date is one (1) year from date of opening. Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. .

Restrictions: For Research Use only

Publications

Titus JA, Haugland R, Sharrow SO et al.: "Texas Red, a hydrophilic, red-emitting fluorophore for use with fluorescein in dual parameter flow microfluorometric and fluorescence microscopic studies." in: J Immunol Methods, Vol. 50, Issue 2, pp. 193-204, 1982 (PubMed).