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Rabbit anti-Goat IgG (Fc Region) Antibody

ELISA, IHC, WB Polyclonal unconjugated
Catalog No. ABIN101247
  • Target See all IgG products
    IgG
    Binding Specificity
    • 2333
    • 498
    • 411
    • 188
    • 129
    • 122
    • 92
    • 29
    • 28
    • 28
    • 15
    • 9
    • 6
    • 6
    • 6
    • 6
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Fc Region
    Reactivity
    • 692
    • 672
    • 558
    • 451
    • 365
    • 239
    • 198
    • 147
    • 137
    • 131
    • 122
    • 98
    • 95
    • 95
    • 92
    • 62
    • 46
    • 33
    • 27
    • 17
    • 12
    • 10
    • 8
    • 5
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Goat
    Host
    • 1716
    • 1473
    • 537
    • 203
    • 118
    • 103
    • 33
    • 31
    • 21
    • 17
    • 15
    • 11
    • 11
    • 1
    Rabbit
    Clonality
    • 3346
    • 164
    • 1
    Polyclonal
    Application
    • 1836
    • 1788
    • 1785
    • 1498
    • 960
    • 934
    • 913
    • 515
    • 234
    • 115
    • 115
    • 111
    • 100
    • 77
    • 59
    • 50
    • 50
    • 49
    • 32
    • 27
    • 25
    • 21
    • 17
    • 16
    • 11
    • 8
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
    Specificity
    IgG F(c)
    Characteristics
    Concentration Definition: by Refractometry
    Purification
    This product was prepared from monospecific antiserum by a delipidation and defibrination.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, Goat IgG, Goat IgG F(c) and Goat Serum.  No reaction was observed against Goat IgG F(ab).
    Immunogen

    Immunogen: Goat IgG F(c) fragment

  • Application Notes

    Immunohistochemistry Dilution: 1:1,000 - 1:5,000

    Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

    ELISA Dilution: 1:20,000 - 1:100,000

    Western Blot Dilution: 1:2,000 - 1:10,000

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 2.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Concentration
    80 mg/mL
    Buffer

    Stabilizer: None

    Preservative: None

    Preservative
    Without preservative
    Storage
    RT,4 °C,-20 °C
    Expiry Date
    12 months
  • Target
    IgG
    Abstract
    IgG Products
    Target Type
    Antibody
    Background

    Synonyms: rabbit anti-Goat IgG F(c) fragment Antibody, rabbit anti-Goat IgG Fc fragment Antibody, rabbit anti Goat IgG Fc

    Background: Anti-Goat IgG F(c) generated in rabbit is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH . Receptors bind the Fc portion of goat IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity.

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