Goat anti-Guinea Pig IgG (Heavy & Light Chain) Antibody
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- Target See all IgG products
- IgG
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Binding Specificity
- Heavy & Light Chain
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Reactivity
- Guinea Pig
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Host
- Goat
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Clonality
- Polyclonal
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Application
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- Specificity
- IgG (H&L)
- Characteristics
- Concentration Definition: by UV absorbance at 280 nm
- Purification
- This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Guinea Pig IgG and Guinea Pig Serum.
- Immunogen
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Immunogen: Guinea Pig IgG whole molecule
- Isotype
- IgG
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- Application Notes
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Immunohistochemistry Dilution: 1:1,000 - 1:5,000
Application Note: Anti-Guinea Pig IgG antibody is suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.
ELISA Dilution: 1:20,000 - 1:100,000
Western Blot Dilution: 1:2,000 - 1:10,000
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Reconstitution Volume: 5.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Concentration
- 10.0 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- RT,4 °C,-20 °C
- Expiry Date
- 12 months
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- Target
- IgG
- Abstract
- IgG Products
- Target Type
- Antibody
- Background
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Synonyms: goat anti-Guinea Pig IgG Antibody, goat anti Guinea Pig IgG
Background: Anti-Guinea Pig IgG Antibody generated in goat detects guinea pig IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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