Rabbit anti-Horse IgG (F(ab')2 Region) Antibody - Preadsorbed
-
- Target See all IgG products
- IgG
-
Binding Specificity
- F(ab')2 Region
-
Reactivity
- Horse
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Application
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- Specificity
- IgG F(ab')2
- Characteristics
- Concentration Definition: by UV absorbance at 280 nm
- Purification
- Preadsorption: Solid phase absorption
- Sterility
- Sterile filtered
- Immunogen
-
Immunogen: Horse IgG F(ab')2 fragment
- Isotype
- IgG
-
Rabbit anti-Horse IgG (Whole Molecule) antibody (HRP)
ELISA, WB Polyclonal IgG HRP
Rabbit anti-Horse IgG (Heavy & Light Chain) antibody (Alkaline Phosphatase (AP)) - PreadsorbedELISA, IHC, WB Polyclonal IgG Alkaline Phosphatase (AP)
Goat anti-Horse IgG (F(ab')2 Region) antibody (HRP)ELISA, IHC, WB, DB, IEM Polyclonal IgG HRP
-
- Application Notes
-
Immunohistochemistry Dilution: 1:1,000 - 1:5,000
Application Note: Anti-Horse IgG F(ab')2 antibody is suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.
ELISA Dilution: 1:20,000 - 1:100,000
Western Blot Dilution: 1:2,000 - 1:10,000
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 2.0 mg/mL
- Buffer
-
Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Expiry Date
- 12 months
-
- Target
- IgG
- Abstract
- IgG Products
- Target Type
- Antibody
- Background
-
Synonyms: Rabbit Anti Horse IgG Fab2 Fragment Antibody, Rabbit Anti-Horse IgG F(ab')2 Antibody, Rabbit Anti Horse IgG Fab2 Antibody
Background: Anti-Horse IgG F(ab')2 Antibody generated in rabbit is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . F(ab')2 Molecules lack the Fc portion of IgG and therefore receptors that bind horse IgG F(c) will not bind horse IgG F(ab')2 Molecules. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
-
