Mouse anti-Human Immunoglobulin G (IgG) Antibody

Antigen: Immunoglobulin G (IgG)

Clonality: Monoclonal (A57H)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN101463

Additional Information

Alternative name: Immunoglobulin G

Immunogen: pFc'- the fragment obtained by pepsin digestion of human IgG1 Fc preparation.

Format: Supernatant

Isotype: IgM kappa

Clone: A57H

Specificity: This antibody reacts with all human IgG isotypes. It stains IgG containing plasma cells and their precursors, including B cell immunoblasts.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Enzymatic predigestion with proteolytic enzymes is recommended for paraffin embedded tissue sections. Positive Control: Tonsil. Cellular localization: Cytoplasm

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as tissue culture supernatant containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications

Publications: Stein, Lennert, Feller et al.: "Immunohistological analysis of human lymphoma: correlation of histological and immunological categories." in: Advances in cancer research, Vol. 42, pp. 67-147, 1985 (PubMed).

Stein, Gerdes, Mason: "The normal and malignant germinal centre." in: Clinics in haematology, Vol. 11, Issue 3, pp. 531-59, 1983 (PubMed).