Goat anti-Mouse IgG (Heavy & Light Chain) Antibody (FITC) - Preadsorbed

Catalog No. ABIN101753

Product Details

Target: IgG

Binding Specificity: Heavy & Light Chain

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: FITC

Application: Flow Cytometry (FACS), Fluorescence Microscopy (FM)

Specificity: IgG (H&L)

Characteristics: Concentration Definition: by UV absorbance at 280 nm

Purification: Preadsorption: Solid phase absorption

Immunogen:

Immunogen: Mouse IgG whole molecule

Isotype: IgG

Labeling Ratio: 6.6

Application Details

Application Notes:

Application Note: Mouse secondary antibody conjugated to FITC is available in a variety of formats. Anti IgG secondary antibody conjugated is suitable for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. Anti mouse IgG FITC is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Flow Cytometry Dilution: 1:1,000 - 1:5,000

IF Microscopy Dilution: 1:500 - 1:2,500

Comment:

Excitation/Emission wavelength: 494 nm/514 nm

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 2.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Product is photosensitive and should be protected from light.

Storage: RT,4 °C,-20 °C

Expiry Date: 12 months

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background:

Synonyms: Goat Anti-Mouse IgG Secondary Antibody fluorescein Conjugated, Goat Anti-Mouse IgG Antibody FITC Conjugated, GAM-FITC, Anti-mouse IgG secondary antibody, anti-mouse IgG Fluorescein conjugated secondary antibody

Background: Anti-Mouse IgG Fluorescein Antibody generated in goat detects reactivity to Mouse IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.