Rabbit anti-Mouse (Murine) IgG (Heavy & Light Chain) Antibody

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Antigen
Epitope
Heavy & Light Chain
4446
2573
1789
1039
928
825
305
272
35
34
31
8
7
7
3
3
2
2
1
1
1
1
1
1
Reactivity
Mouse (Murine)
2684
2654
2280
1644
1387
741
607
423
364
338
330
324
307
258
168
148
126
114
41
27
25
22
12
11
9
8
6
5
5
4
4
4
4
4
4
4
4
3
3
3
2
2
1
1
1
1
1
Host
Rabbit
6579
4499
1779
792
637
372
87
52
50
9
8
5
5
4
2
1
1
Clonality
Polyclonal
Application
Dot Blot (DB), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
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Immunogen Anti-Mouse IgG was produced by repeated immunization with mouse IgG whole molecule in rabbit
Isotype IgG
Specificity IgG (H&L)
Characteristics Anti-Mouse antibody generated in rabbit detects specifically mouse IgG (H&L). This secondary antibody anti-Mouse is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Concentration Definition: by UV absorbance at 280 nm
Sterility Sterile filtered
Background Synonyms: Mouse IgG (H&L) Antibody, Rabbit-a-Mouse IgG (H&L), Mouse IgG (H&L) Antibody in rabbit, Goat IgG (H&L) Secondary Antibody.
Research Area Immunology, Secondary Antibodies
Application Notes Antibody Anti-Mouse IgG (H&L) is suitable for immunoblotting (western or dot blot), ELISA, and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Restrictions For Research Use only
Format Liquid
Concentration 2.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Background publications Titus, Haugland, Sharrow, Segal: "Texas Red, a hydrophilic, red-emitting fluorophore for use with fluorescein in dual parameter flow microfluorometric and fluorescence microscopic studies." in: Journal of immunological methods, Vol. 50, Issue 2, pp. 193-204, 1982 (PubMed).