USP6 antibody (AA 142-155)

Catalog No. ABIN1019674

Product Details anti-USP6 Antibody

Target: USP6 (Ubiquitin Specific Peptidase 6 (USP6))

Binding Specificity: AA 142-155

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This USP6 antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Purpose: USP6 (aa142-155)

Sequence: HHIDLDVRTT LRNH

Specificity: This antibody is expected NOT to cross react to USP32 or to the TBC1D3 proteins.

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-HHIDLDVRTTLRNH, from the internal region (near N Terminus) of the protein sequence according to NP_004496.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Preliminary experiments in Human Testis, Prostate and Placenta lysates gave no specific signal but low background at antibody concentration up to 1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:4000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 and U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration:

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for USP6

Target: USP6 (Ubiquitin Specific Peptidase 6 (USP6))

Alternative Name: USP6 (USP6 Products)

Background: USP6, ubiquitin specific peptidase 6 (Tre-2 oncogene), HRP1, TRE17, TRE2, Tre-2, USP6-short, OTTHUMP00000125301, deubiquitinating enzyme 6, hyperpolymorphic gene 1, proto-oncogene TRE-2, tre-2 oncogene, ubiquitin carboxyl-terminal hydrolase 6, ubiquitin s

Gene ID: 9098, 237898

NCBI Accession: NP_004496