SRY (Sex Determining Region Y)-Box 2 (SOX2) antibody

Antigen: SRY (Sex Determining Region Y)-Box 2 (SOX2)

Synonyms: ANOP3, MCOPS3, MGC2413, RGD1565646, SOX2, MGC148683, lcc, ysb, Sox-2

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC)

Catalog no.: ABIN1020451

Additional Information

Alternative name: SRY (sex determining region Y)-box 2, SOX2

Immunogen: A synthetic peptide (conjugated with KLH) corresponding to the sequence of SOX2.

Format: Liquid

Isotype: Ig

Description: Background: SRY (sex determining region Y)-box 2, also known as SOX2, is a transcription factor that is essential to maintain self-renewal of undifferentiated embryonic stem cells. SOX2 gene encodes a 317-amino acid protein, 1 and lies in an intron of the SOX2OT (SOX2 overlapping transcript) gene with single-exon. 2 SOX2 is expressed early in the embryonic stem cells of the blastocyst and later in the neural stem cells. It is a member of the SOX family of proteins that carry a DNA-binding high-mobility group (HMG) domain and additional domains that regulate embryonic development and cell fate determinations. Mutations in SOX2 can cause not only anophthalmia, but also aplasia of the optic nerve, chiasm and optic tract, as well as modest bilateral sensorineural hearing loss, and global developmental delay, underscoring the importance of SOX2 in early human eye and brain development. 3 And the ectopic expression of SOX2 may be related to abnormal differentiation of colorectal cancer cells.

Application Details

Application Notes: Western Blot (0.5-1myg/ml) Immunohistochemistry (2.5myg/ml) Immunocytochemistry (10-25myg/ml) Other applications have not been tested. The optimal dilutions should be determined by end user.

Purification: affinity purified

Buffer: In TBS (pH7.4), 40% Glycerol

Preservative: 0.5% BSA, 0.05% Sodium Azide

Storage (Long Term): Aliquot and store at -20°C for long term (at least one year). Avoid repeated freeze and thaw cycles.

Storage (Short Term): Store at 4°C for short term (1 week).

Restrictions: For Research Use Only

Images

anti-SRY (Sex Determining Region Y)-Box 2 (SOX2) antibody

anti-SRY (Sex Determining Region Y)-Box 2 (SOX2) antibody (Image 2)

Publications

Product: Stevanovic, Zuffardi, Collignon et al.: "The cDNA sequence and chromosomal location of the human SOX2 gene." in: Mammalian genome : official journal of the International Mammalian Genome Society, Vol. 5, Issue 10, pp. 640-2, 1995 (PubMed).

Fantes, Ragge, Lynch et al.: "Mutations in SOX2 cause anophthalmia." in: Nature genetics, Vol. 33, Issue 4, pp. 461-3, 2003 (PubMed).

Hagstrom, Pauer, Reid et al.: "SOX2 mutation causes anophthalmia, hearing loss, and brain anomalies." in: American journal of medical genetics. Part A, Vol. 138A, Issue 2, pp. 95-8, 2005 (PubMed).

Tani, Akiyama, Fukamachi et al.: "Transcription factor SOX2 up-regulates stomach-specific pepsinogen A gene expression." in: Journal of cancer research and clinical oncology, Vol. 133, Issue 4, pp. 263-9, 2007 (PubMed).