Rabbit anti-Mouse IgG1 (Heavy Chain) Antibody (FITC) - Preadsorbed

Catalog No. ABIN102336

Product Details

Target: IgG1

Binding Specificity: Heavy Chain

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: FITC

Application: Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)

Specificity: IgG1

Characteristics: Concentration Definition: by UV absorbance at 280 nm

Purification: Preadsorption: Solid phase absorption

Immunogen:

Immunogen: Mouse IgG1 heavy chain

Isotype: IgG

Labeling Ratio: 3.9

Application Details

Application Notes:

Application Note: Mouse IgG1 secondary antibody conjugated to FITC is available in a variety of formats. Anti Mouse IgG1 secondary antibody conjugate is suitable for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This IgG1 antibody is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

FLISA Dilution: 1:10,000 - 1:50,000

Flow Cytometry Dilution: User Optimized

IF Microscopy Dilution: 1:500 - 1:2,500

Comment:

Excitation/Emission wavelength: 494 nm/514 nm

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Product is photosensitive and should be protected from light.

Storage: RT,4 °C,-20 °C

Expiry Date: 12 months

Target Details

Target: IgG1

Abstract: IgG1 Products

Target Type: Antibody

Background:

Synonyms: Rabbit Anti-Mouse IgG1 (Gamma 1 chain) Antibody fluorescein Conjugation, Rabbit Anti-Mouse IgG1 FITC Conjugated Antibody

Background: Anti-Mouse IgG1 Fluorescein Antibody generated in rabbit detects reactivity to Mouse IgG1 (Gamma 1 chain). Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG1 chain constitutes 66 % of the IgG subclass and has a high affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.