Rabbit anti-Mouse IgG3 (Heavy Chain) Antibody (Biotin) - Preadsorbed

Catalog No. ABIN102435

Product Details

Target: IgG3

Binding Specificity: Heavy Chain

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: Biotin

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Specificity: IgG3

Characteristics: Concentration Definition: by UV absorbance at 280 nm

Purification: Preadsorption: Solid phase absorption

Immunogen:

Immunogen: Mouse IgG3 heavy chain

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:500 - 1:2,000

Application Note: Secondary antibody reagents are ideal for ELISA, western blotting, Immunohistochemistry, Fluorescence Microscopy, Flow Cytometry as well as other antibody detection methods.

ELISA Dilution: 1:8,000 - 1:32,000

Western Blot Dilution: 1:1,000 - 1:5,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Aliquot to Avoid repeated freezing and thawing.

Storage: RT,4 °C,-20 °C

Expiry Date: 12 months

Target Details

Target: IgG3

Abstract: IgG3 Products

Target Type: Antibody

Background:

Synonyms: Rabbit Anti-Mouse IgG3 (Gamma 3 chain) Biotin Conjugated Antibody, Rabbit Anti Mouse IgG3 Antibody Biotin Conjugation

Background: Anti-Mouse IgG3 Biotin Antibody generated in rabbit detects reactivity to Mouse IgG3 (Gamma 3 chain). Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG3 comprises almost 10 % of the IgG subclass and has a high affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.