Mouse anti-Human Immunoglobulin lambda (IgL) Antibody

Antigen: Immunoglobulin lambda (IgL)

Clonality: Monoclonal (HP6054)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN102518

Additional Information

Alternative name: Immunoglobulin Lambda

Immunogen: Purified human IgG myeloma proteins covalently linked to polyaminostyrene micro beads.

Format: Supernatant (prediluted)

Isotype: IgG2a  (Matching secondary antibodies)

Clone: HP6054

Specificity: This antibody stains B cell follicles in human lymphoid tissues. The mantle zone shows a mosaic pattern of labeling while the germinal centers show a coarse meshwork pattern of staining. Lambda light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non-Hodgkins lymphomas. This antibody does not react with rat.

Application Details

Application Notes: Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Tonsil. Cellular localization: Cytoplasm

Concentration: 1 to 4 ug/ml

Buffer: This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications

Publications: Jefferis, Reimer, Skvaril et al.: "Evaluation of monoclonal antibodies having specificity for human IgG subclasses: results of the 2nd IUIS/WHO collaborative study." in: Immunology letters, Vol. 31, Issue 2, pp. 143-68, 1992 (PubMed).

Reimer, Phillips, Aloisio et al.: "Evaluation of thirty-one mouse monoclonal antibodies to human IgG epitopes." in: Hybridoma, Vol. 3, Issue 3, pp. 263-75, 1985 (PubMed).

Black, McDougal, Holman et al.: "Cross-reactivity of 75 monoclonal antibodies to human immunoglobulin with sera of non-human primates." in: Immunology letters, Vol. 37, Issue 2-3, pp. 207-13, 1994 (PubMed).