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Mouse anti-Human Immunoglobulin M (IgM) Antibody

Antigen

Immunoglobulin M (IgM)

Clonality Monoclonal (R1-69)
Host
Alternatives

Mouse

Reactivity
Alternatives

Human

Conjugate
Alternatives Un-conjugated
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
2 references available
Catalog no. ABIN102597
Quantity 6ml  (1 to 4 ug/ml)  (Variants)
Price 162.00 $   Plus shipping costs $35.00
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Additional Information

Alternative name Immunoglobulin M
Format Supernatant (prediluted)
Isotype IgG1 kappa
Clone R1-69
Specificity This antibody reacts with the heavy chain present in all types of human IgM. It stains B cell follicles in human lymphoid tissues. In lymphoid tissues, IgM containing plasma cells stain strongly but immune complexes are usually undetectable.

Application Details

Application Notes Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Proteolytic treatment is required prior to immunostaining of formalin fixed paraffin embedded tissue sections. Positive Control: Tonsil. Cellular localization: Cytoplasmic
Concentration 1 to 4 ug/ml
Buffer This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required.
Storage Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
Restrictions For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications

Publications Pallesen, Kerndrup, Ellegaard: "Further evidence for the B-cell nature of hairy cells. A study using immunostaining of splenic tissue with a wide panel of monoclonal antibodies." in: Blut, Vol. 49, Issue 5, pp. 395-403, 1984 (PubMed).

Stein, Gerdes, Mason: "The normal and malignant germinal centre." in: Clinics in haematology, Vol. 11, Issue 3, pp. 531-59, 1983 (PubMed).

Alternatives

Alternatives for antigen "Immunoglobulin M (IgM)", type "Secondary Antibodies"
Hosts Goat (195), Rabbit (129), Mouse (34), Rat (14), Chicken (1)
Reactivities Human (112), Mouse (Murine) (100), Rat (Rattus) (51), Monkey (19), Chicken (18), Ferret (13), Sheep (Ovine) (8), Cat (Feline) (7), Cow (Bovine) (7), Dog (Canine) (7), Horse (Equine) (7), Rabbit (7), Goat (6), Pig (Porcine) (6), Hamster (4), Syrian Hamster (2), Donkey (1), Guinea Pig (1), Turkey (1)
Conjugates FITC (67), HRP (64), Biotin (45), Alkaline Phosphatase (AP) (40), Texas Red (TR) (24), TRITC (20), Rhodamine (14), PE (8), DyLight 488 (6), Peroxidase (POD) (5), APC (4), Colloidal Gold (15 OD) (2), Colloidal Gold (50 OD) (2), RPE (2), SPRD (2), Cy5 (1), DyLight 405 (1), DyLight 594 (1), DyLight 680 (1), DyLight 800 (1), PE,Cy7 (1)
Epitopes Heavy chain (100), H&L (18), Fc5m (7), Whole (7), Fc5u (3), Fc- (2), Fc5u specific (1), Fcu (1)