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Rabbit anti-Human IgM (Heavy Chain) Antibody

ELISA, IHC, WB Polyclonal unconjugated
Catalog No. ABIN102631
  • Target See all IgM products
    IgM
    Binding Specificity
    • 141
    • 117
    • 87
    • 41
    • 17
    • 8
    • 7
    • 1
    • 1
    Heavy Chain
    Reactivity
    • 188
    • 130
    • 65
    • 39
    • 32
    • 26
    • 22
    • 21
    • 16
    • 15
    • 15
    • 14
    • 13
    • 13
    • 12
    • 4
    • 2
    Human
    Host
    • 321
    • 187
    • 73
    • 15
    • 14
    • 5
    • 4
    • 1
    • 1
    Rabbit
    Clonality
    • 495
    • 78
    Polyclonal
    Application
    • 332
    • 249
    • 238
    • 208
    • 143
    • 139
    • 64
    • 63
    • 47
    • 47
    • 46
    • 42
    • 38
    • 29
    • 20
    • 18
    • 13
    • 6
    • 5
    • 5
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
    Specificity
    IgM µ chain
    Characteristics
    Concentration Definition: by Refractometry
    Purification
    This product was prepared from monospecific antiserum by a delipidation and defibrination.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, Human IgM and Human Serum.  No reaction was observed against Human IgG.
    Immunogen

    Immunogen: Human IgM mu heavy chain

  • Application Notes

    Immunohistochemistry Dilution: 1:1,000 - 1:5,000

    Application Note: Anti-Human IgM (mu heavy chain) is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

    ELISA Dilution: 1:20,000 - 1:100,000

    Western Blot Dilution: 1:2,000 - 1:10,000

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 2.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Concentration
    90 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Storage
    RT,4 °C,-20 °C
    Expiry Date
    12 months
  • Target
    IgM
    Abstract
    IgM Products
    Target Type
    Antibody
    Background

    Synonyms: rabbit anti-Human IgM mu chain Antibody, rabbit anti Human IgM mu

    Background: Anti-Human IgM (mu heavy chain) generated in rabbit detects specifically Human IgM mu heavy chain. Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together. Anti-Human IgM mu chain antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.

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