Goat anti-Mouse IgM (Heavy & Light Chain) Antibody (Alkaline Phosphatase (AP)) - Preadsorbed

Catalog No. ABIN102675

Product Details

Target: IgM

Binding Specificity: Heavy & Light Chain

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: Alkaline Phosphatase (AP)

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Specificity: IgM µ chain

Characteristics: Concentration Definition: by UV absorbance at 280 nm

Purification: Preadsorption: Solid phase absorption

Sterility: Sterile filtered

Immunogen:

Immunogen: Mouse IgM whole molecule

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:200 - 1:1,000

Application Note: Mouse IgM (mu chain) alkaline phosphatase conjugated Antibody is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring extremely low background levels, lot-to-lot consistency, high titer and specificity.

ELISA Dilution: 1:4,500 - 1:8,000

Western Blot Dilution: 1:500 - 1:2,500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.1 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Dilute only prior to immediate use
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Expiry Date: 12 months

Target Details

Target: IgM

Abstract: IgM Products

Target Type: Antibody

Background:

Synonyms: goat anti-Mouse IgM (Mu Chain) alkaline phosphatase Conjugated Antibody, goat anti-Mouse IgM Mu Chain Antibody alk phos Conjugation

Background: Anti-Mouse IgM (mu chain) alkaline phosphatase conjugated antibody generated in goat detects specifically mouse IgM. Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approximate molecular weight of IgM is 900 kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum. Anti-Mouse IgM antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.