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CD63 antibody (CD63 Molecule) (Biotin)

Details for Product anti-CD63 Antibody No. ABIN1027697, Supplier: Log in to see
Antigen
  • C75951
  • ME491
  • Tspan30
  • LAMP-3
  • MLA1
  • OMA81H
  • TSPAN30
  • TSP29FA
Reactivity
Human
277
42
20
3
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Host
Mouse
241
68
12
2
Clonality (Clone)
Monoclonal ()
Conjugate
This CD63 antibody is conjugated to Biotin
37
25
21
20
7
4
4
3
3
2
2
2
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
195
103
67
51
51
50
47
30
5
4
4
4
2
2
2
2
2
2
1
1
Supplier
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Immunogen HPB-ALL T cell line
Clone MEM-259
Isotype IgG1
Specificity The antibody MEM-259 reacts with CD63 (LAMP-3), a 40-60 kDa tetraspan glycoprotein expressed by granulocytes, platelets, T cells, monocytes/macrophages and endothelial cells. Cell surface exposition of CD63 is usually activation-dependent.
Characteristics The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
Alternative Name CD63 (CD63 Antibody Abstract)
Background CD63 (LAMP-3, lysosome-associated membrane protein-3), a glycoprotein of tetraspanin family, is present in late endosomes, lysosomes and secretory vesicles of various cell types. It is also present in the plasma membrane, usually following cell activation. Hence, it has become an widely used basophil activation marker. In mast cells, however, CD63 exposition does not need their activation. CD63 interacts with integrins and affects phagocytosis and cell migration, it is also involved in H/K-ATPase trafficking regulation of ROMK1 channels. CD63 also serves as a T-cell costimulation molecule. Expression of CD63 can be used for predicting the prognosis in earlier stages of carcinomas.
Research Area CD Antigens, Surface Receptors of Immune Cells
Application Notes Biotinylated antibody is designed for indirect immunofluorescence analysis by Flow Cytometry. Working concentrations should be determined by the investigator.

Working concentrations should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store at 2-8 °C. Do not use after expiration date stamped on vial label.
Supplier Images
Immunofluorescence (IF) image for anti-CD63 antibody (CD63 Molecule)  (Biotin) (ABIN1027697) Immunofluorescence staining of human skin fibroblasts with anti-CD63 (MEM-259 ; green...
Immunofluorescence (IF) image for anti-CD63 antibody (CD63 Molecule)  (Biotin) (ABIN1027697) Immunofluorescence staining of human skin fibroblasts with anti-CD63 (MEM-259 ; green...
Immunofluorescence (IF) image for anti-CD63 antibody (CD63 Molecule)  (Biotin) (ABIN1027697) Immunofluorescence staining of CD63 in human HeLa cell line using anti-CD63
Flow Cytometry (FACS) image for anti-CD63 antibody (CD63 Molecule)  (Biotin) (ABIN1027697) Flow cytometry analysis of peripheral blood lymphocytes from a patient with allergy t...
Product cited in: Heneberg, Riegerová, Kučera: "Pimecrolimus Is a Potent Inhibitor of Allergic Reactions to Hymenopteran Venom Extracts and Birch Pollen Allergen In Vitro." in: PLoS ONE, Vol. 10, Issue 11, pp. e0142953, 2015 (PubMed).

Cerny, Feng, Yu et al.: "The small chemical vacuolin-1 inhibits Ca(2+)-dependent lysosomal exocytosis but not cell resealing." in: EMBO reports, Vol. 5, Issue 9, pp. 883-8, 2004 (PubMed).

Background publications Lin, Kamsteeg, Zhang et al.: "Expression of tetraspan protein CD63 activates protein-tyrosine kinase (PTK) and enhances the PTK-induced inhibition of ROMK channels." in: The Journal of biological chemistry, Vol. 283, Issue 12, pp. 7674-81, 2008 (PubMed).

Israels, McMillan-Ward: "CD63 modulates spreading and tyrosine phosphorylation of platelets on immobilized fibrinogen." in: Thrombosis and haemostasis, Vol. 93, Issue 2, pp. 311-8, 2005 (PubMed).

Pfistershammer, Majdic, Stöckl et al.: "CD63 as an activation-linked T cell costimulatory element." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 173, Issue 10, pp. 6000-8, 2004 (PubMed).

Grützkau, Smorodchenko, Lippert et al.: "LAMP-1 and LAMP-2, but not LAMP-3, are reliable markers for activation-induced secretion of human mast cells." in: Cytometry. Part A : the journal of the International Society for Analytical Cytology, Vol. 61, Issue 1, pp. 62-8, 2004 (PubMed).

Mantegazza, Barrio, Moutel et al.: "CD63 tetraspanin slows down cell migration and translocates to the endosomal-lysosomal-MIICs route after extracellular stimuli in human immature dendritic cells." in: Blood, Vol. 104, Issue 4, pp. 1183-90, 2004 (PubMed).