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CD8 antibody (CD8a Molecule)

Details for Product anti-CD8A Antibody No. ABIN1027698, Supplier: Log in to see
Antigen
  • BB154331
  • CD8
  • cd8
  • Leu2
  • LOC100136450
  • Ly-2
  • Ly-35
  • Ly-B
  • Lyt-2
  • MAL
  • p32
  • RHACD8-4
  • zgc:136643
Alternatives
anti-Rat (Rattus) CD8 antibody for Immunohistochemistry (Frozen Sections)
Reactivity
Rat (Rattus)
1026
538
163
73
47
41
22
22
20
20
19
16
15
13
13
12
12
12
11
11
10
10
8
3
3
2
2
2
2
1
1
1
1
Host
Mouse
1276
510
193
1
Clonality (Clone)
Monoclonal ()
Conjugate
This CD8 antibody is un-conjugated
258
217
138
81
56
44
41
37
33
29
28
28
26
25
24
24
24
24
20
20
19
17
16
14
12
11
10
10
10
6
6
4
4
4
4
3
3
3
3
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Functional Studies (Func), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
1617
439
351
336
268
262
239
235
199
38
37
25
23
18
14
10
10
7
7
6
6
6
5
4
4
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Supplier
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Immunogen High Mw glycoproteins from rat thymocytes
Clone OX-8
Isotype IgG1
Specificity The mouse monoclonal antibody OX-8 recognizes the hinge-like membrane-proximal domain of rat CD8a (32-34 kDa, alpha chain of the CD8 antigen).
Purification Purified from cell culture supernatant by protein-A affinity chromatography.
Purity > 95 % (by SDS-PAGE)
Alternative Name CD8A (CD8A Antibody Abstract)
Background The CD8a (CD8 alpha) subunit of CD8 T cell coreceptor is expressed in CD8 alpha/beta heterodimers on majority of MHC I-restricted conventional T cells and thymocytes and in CD8 alpha/alpha homodimers on subsets of memory T cells, intraepithelial lymphocytes, NK cells, macrophages and dendritic cells. Regulation of CD8 beta level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 alpha/beta but not alpha/alpha dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.
Research Area Surface Receptors of Immune Cells, CD Antigens, Major Histocompatibility Complex (MHC)
Pathways TCR Signaling
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Product cited in: Pino, OSullivan-Murphy, Lidstone, Yang, Lipson, Jurczyk, diIorio, Brehm, Mordes, Greiner, Rossini, Bortell: "CHOP mediates endoplasmic reticulum stress-induced apoptosis in Gimap5-deficient T cells." in: PLoS ONE, Vol. 4, Issue 5, pp. e5468, 2009 (PubMed).

Abe, Urakami, Ostanin, Zibari, Hayashida, Kitagawa, Grisham: "Induction of Foxp3-expressing regulatory T-cells by donor blood transfusion is required for tolerance to rat liver allografts." in: PLoS ONE, Vol. 4, Issue 11, pp. e7840, 2009 (PubMed).

Katsumata, Harigai, Sugiura, Kawamoto, Kawaguchi, Matsumoto, Kohyama, Soejima, Kamatani, Hara: "Attenuation of experimental autoimmune myositis by blocking ICOS-ICOS ligand interaction." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 6, pp. 3772-9, 2007 (PubMed).

Ishida, Usui, Yamashiro, Kaji, Amano, Ogura, Hida, Oguchi, Ambati, Miller, Gragoudas, Ng, DAmore, Shima, Adamis: "VEGF164-mediated inflammation is required for pathological, but not physiological, ischemia-induced retinal neovascularization." in: The Journal of experimental medicine, Vol. 198, Issue 3, pp. 483-9, 2003 (PubMed).

Mitnacht, Bischof, Torres-Nagel, Hünig: "Opposite CD4/CD8 lineage decisions of CD4+8+ mouse and rat thymocytes to equivalent triggering signals: correlation with thymic expression of a truncated CD8 alpha chain in mice but not rats." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 2, pp. 700-7, 1998 (PubMed).

Hirji, Lin, Befus: "A novel CD8 molecule expressed by alveolar and peritoneal macrophages stimulates nitric oxide production." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 4, pp. 1833-40, 1997 (PubMed).

Torres-Nagel, Kraus, Brown, Tiefenthaler, Mitnacht, Williams, Hünig: "Differential thymus dependence of rat CD8 isoform expression." in: European journal of immunology, Vol. 22, Issue 11, pp. 2841-8, 1992 (PubMed).

Barclay: "The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues." in: Immunology, Vol. 42, Issue 4, pp. 593-600, 1981 (PubMed).