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CD8 antibody (CD8a Molecule)

Details for Product anti-CD8A Antibody No. ABIN1027698, Supplier: Log in to see
Antigen
  • BB154331
  • CD8
  • cd8
  • Leu2
  • LOC100136450
  • Ly-2
  • Ly-35
  • Ly-B
  • Lyt-2
  • MAL
  • p32
  • RHACD8-4
  • zgc:136643
Alternatives
anti-Rat (Rattus) CD8 antibody for Immunohistochemistry (Frozen Sections)
Reactivity
Rat (Rattus)
1059
532
166
68
47
40
25
22
20
20
19
16
15
13
12
12
11
11
10
10
8
8
5
3
3
2
2
2
2
1
1
1
Host
Mouse
1285
512
206
Clonality (Clone)
Monoclonal ()
Conjugate
This CD8 antibody is un-conjugated
259
217
142
86
52
42
40
39
30
30
29
28
27
26
25
25
25
25
25
21
21
18
18
17
11
10
10
10
8
6
6
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4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
1617
451
356
342
284
282
254
238
214
36
33
25
23
17
14
10
7
6
6
6
5
4
4
4
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Supplier
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Immunogen High Mw glycoproteins from rat thymocytes
Clone OX-8
Isotype IgG1
Specificity The mouse monoclonal antibody OX-8 recognizes the hinge-like membrane-proximal domain of rat CD8a (32-34 kDa, alpha chain of the CD8 antigen).
Purification Purified from cell culture supernatant by protein-A affinity chromatography.
Purity > 95 % (by SDS-PAGE)
Alternative Name CD8A (CD8A Antibody Abstract)
Background The CD8a (CD8 alpha) subunit of CD8 T cell coreceptor is expressed in CD8 alpha/beta heterodimers on majority of MHC I-restricted conventional T cells and thymocytes and in CD8 alpha/alpha homodimers on subsets of memory T cells, intraepithelial lymphocytes, NK cells, macrophages and dendritic cells. Regulation of CD8 beta level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 alpha/beta but not alpha/alpha dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.
Pathways TCR Signaling
Application Notes Functional Application: blocking

Working concentrations should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8 °C. Do not use after expiration date stamped on vial label.
Product cited in: Pino, OSullivan-Murphy, Lidstone, Yang, Lipson, Jurczyk, diIorio, Brehm, Mordes, Greiner, Rossini, Bortell: "CHOP mediates endoplasmic reticulum stress-induced apoptosis in Gimap5-deficient T cells." in: PLoS ONE, Vol. 4, Issue 5, pp. e5468, 2009

Abe, Urakami, Ostanin, Zibari, Hayashida, Kitagawa, Grisham: "Induction of Foxp3-expressing regulatory T-cells by donor blood transfusion is required for tolerance to rat liver allografts." in: PLoS ONE, Vol. 4, Issue 11, pp. e7840, 2009

Katsumata, Harigai, Sugiura, Kawamoto, Kawaguchi, Matsumoto, Kohyama, Soejima, Kamatani, Hara: "Attenuation of experimental autoimmune myositis by blocking ICOS-ICOS ligand interaction." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 6, pp. 3772-9, 2007

Ishida, Usui, Yamashiro, Kaji, Amano, Ogura, Hida, Oguchi, Ambati, Miller, Gragoudas, Ng, DAmore, Shima, Adamis: "VEGF164-mediated inflammation is required for pathological, but not physiological, ischemia-induced retinal neovascularization." in: The Journal of experimental medicine, Vol. 198, Issue 3, pp. 483-9, 2003

Mitnacht, Bischof, Torres-Nagel, Hünig: "Opposite CD4/CD8 lineage decisions of CD4+8+ mouse and rat thymocytes to equivalent triggering signals: correlation with thymic expression of a truncated CD8 alpha chain in mice but not rats." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 2, pp. 700-7, 1998

Hirji, Lin, Befus: "A novel CD8 molecule expressed by alveolar and peritoneal macrophages stimulates nitric oxide production." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 4, pp. 1833-40, 1997

Torres-Nagel, Kraus, Brown, Tiefenthaler, Mitnacht, Williams, Hünig: "Differential thymus dependence of rat CD8 isoform expression." in: European journal of immunology, Vol. 22, Issue 11, pp. 2841-8, 1992

Barclay: "The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues." in: Immunology, Vol. 42, Issue 4, pp. 593-600, 1981