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DNA (Cytosine-5)-Methyltransferase 1 (DNMT1) (AA 620-950) antibody

Details for Product No. ABIN1027706, Supplier: Log in to see
Antigen
  • cb1075
  • cb983
  • dmnt1
  • fb05h01
  • mta
  • wu:fb05h01
  • ADCADN
  • AIM
  • CXXC9
  • DNMT
  • HSN1E
  • MCMT
  • DDM2
  • DECREASED DNA METHYLATION 2
  • DMT01
  • DMT1
  • DNA METHYLTRANSFERASE
  • DNA METHYLTRANSFERASE 01
  • DNA METHYLTRANSFERASE 1
  • MET2
  • METHYLTRANSFERASE 2
  • METHYLTRANSFERASE I
  • METI
  • methyltransferase 1
  • Cxxc9
  • Dnmt
  • Dnmt1o
  • MTase
  • Met-1
  • Met1
  • MommeD2
  • m.MmuI
  • DNA (cytosine-5-)-methyltransferase 1
  • DNA (cytosine-5)-methyltransferase 1
  • DNA methyltransferase (cytosine-5) 1
  • DNMT1
  • dnmt1
  • Dnmt1
  • MET1
Epitope
AA 620-950
66
43
35
22
21
20
19
16
15
15
15
11
9
7
7
7
6
6
5
4
4
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Fish, Human, Mouse (Murine), Zebrafish
430
182
94
73
39
26
8
8
6
5
5
3
2
2
2
1
1
1
1
Host
Mouse
268
136
20
8
3
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
24
24
21
19
18
18
6
5
5
5
5
5
5
5
5
5
5
5
4
4
4
4
4
4
4
4
4
3
2
1
1
1
1
1
1
1
1
1
1
Application
Chromatin Immunoprecipitation (ChIP), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
284
139
118
112
88
73
53
53
51
38
37
16
6
5
3
2
2
2
2
1
Options
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Immunogen Raised against a partial protein corresponding to amino acids 620-950 of human DNMT1
Clone 4G11-C7
Isotype IgG1 kappa
Specificity Detects ~180 kDa. It will cross-react with mouse DNMT1.
Purification Protein G Purified
Alternative Name DNMT1 (DNMT1 Antibody Abstract)
Background Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Dnmt1 co-purifies with the retinoblastoma (Rb) tumour suppressor gene product, E2F1, and HDAC1. Dnmt1 also cooperates with Rb to repress transcription from promoters containing E2F-binding sites suggesting a link between DNA methylation, histone deacetylase and sequence-specific DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells (1-6).
Gene ID 1786
NCBI Accession NP_001370
UniProt P26358
Research Area DNA/RNA
Application Notes
  • WB (1:500)
  • IHC (1:500)
  • optimal dilutions for assays should be determined by the user.
Comment

2 μg/ml of SMC-201 was sufficient for detection of Dnmt1 in 10 μg of mouse ES cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
Western Blotting (WB) image for anti-DNA (Cytosine-5)-Methyltransferase 1 (DNMT1) (AA 620-950) antibody (ABIN1027706) Dnmt1, 18ug H1299 lysate 1 in1000 Western Blotting.
Background publications Robertson, Ait-Si-Ali, Yokochi, Wade, Jones, Wolffe: "DNMT1 forms a complex with Rb, E2F1 and HDAC1 and represses transcription from E2F-responsive promoters." in: Nature genetics, Vol. 25, Issue 3, pp. 338-42, 2000 (PubMed).

Xie, Wang, Okano, Nogami, Li, He, Okumura, Li: "Cloning, expression and chromosome locations of the human DNMT3 gene family." in: Gene, Vol. 236, Issue 1, pp. 87-95, 1999 (PubMed).

Okano, Bell, Haber, Li: "DNA methyltransferases Dnmt3a and Dnmt3b are essential for de novo methylation and mammalian development." in: Cell, Vol. 99, Issue 3, pp. 247-57, 1999 (PubMed).

Reik, Kelsey, Walter: "Dissecting de novo methylation." in: Nature genetics, Vol. 23, Issue 4, pp. 380-2, 1999 (PubMed).

Yen, Vertino, Nelkin, Yu, el-Deiry, Cumaraswamy, Lennon, Trask, Celano, Baylin: "Isolation and characterization of the cDNA encoding human DNA methyltransferase." in: Nucleic acids research, Vol. 20, Issue 9, pp. 2287-91, 1992 (PubMed).

Bestor, Laudano, Mattaliano, Ingram: "Cloning and sequencing of a cDNA encoding DNA methyltransferase of mouse cells. The carboxyl-terminal domain of the mammalian enzymes is related to bacterial restriction methyltransferases." in: Journal of molecular biology, Vol. 203, Issue 4, pp. 971-83, 1989 (PubMed).