DNA (Cytosine-5)-Methyltransferase 1 (DNMT1) (AA 637-650) antibody

Details for Product No. ABIN1027706
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Antigen
Synonyms
cb1075, cb983, dmnt1, fb05h01, mta, wu:fb05h01, ADCADN, AIM, CXXC9, DNMT, HSN1E, MCMT, DDM2, DECREASED DNA METHYLATION 2, DMT01, DMT1, DNA METHYLTRANSFERASE, DNA METHYLTRANSFERASE 01, DNA METHYLTRANSF ... show more
cb1075, cb983, dmnt1, fb05h01, mta, wu:fb05h01, ADCADN, AIM, CXXC9, DNMT, HSN1E, MCMT, DDM2, DECREASED DNA METHYLATION 2, DMT01, DMT1, DNA METHYLTRANSFERASE, DNA METHYLTRANSFERASE 01, DNA METHYLTRANSFERASE 1, MET2, METHYLTRANSFERASE 2, METHYLTRANSFERASE I, METI, methyltransferase 1, Cxxc9, Dnmt, Dnmt1o, MTase, Met-1, Met1, MommeD2, m.MmuI show less
Epitope
AA 637-650
(22), (20), (17), (16), (13), (13), (12), (12), (12), (12), (11), (9), (7), (6), (6), (6), (5), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Zebrafish
(230), (64), (57), (52), (42), (34), (24), (6), (2), (1), (1), (1), (1), (1), (1)
Host
Mouse
(172), (50), (10), (7), (2)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(13), (13), (11), (8), (8), (8), (5), (5), (5), (5), (5), (5), (5), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application
Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP)
(148), (65), (57), (50), (44), (41), (41), (38), (12), (12), (7), (7), (6), (1), (1)
Pubmed 6 references available
Quantity 100 μg
Options
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Catalog No. ABIN1027706
383.90 $
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Immunogen Raised against a synthetic peptide corresponding to AA 637-650 of human DNMT1 (Gene
Clone 4G11-C7
Isotype IgG1 kappa
Specificity It will cross-react with mouse DNMT1.
Cross-Reactivity (Details) It will cross-react with mouse DNMT1.
Purification Protein G Purified
Alternative Name DNMT1
Background Synonyms:
DNA Mtase, DNMT, MCMT, DNA methyltransferase 1
Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylationof genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 familymembers, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Dnmt1 co-purifies with the retinoblastoma (Rb) tumour suppressor gene product, E2F1, and HDAC1. Dnmt1 also cooperates with Rb to repress transcription from promoters containing E2F-binding sites suggesting a link between DNA methylation, histone deacetylase and sequence-specific DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells.
Gene ID 1786
NCBI Accession NP_001370
UniProt P26358
Research Area DNA/RNA, Cell Signaling, Organelles
Application Notes Recommended Dilution: 2-4 µg/mL (WB), 1-2 µg/mL (IHC)
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer PBS, 0.05 % BSA, 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-DNA (Cytosine-5)-Methyltransferase 1 (DNMT1) (AA 637-650) antibody Dnmt1, 18ug H1299 lysate 1 in1000 Western Blotting.
Background publications Yen, Vertino, Nelkin et al.: "Isolation and characterization of the cDNA encoding human DNA methyltransferase." in: Nucleic acids research, Vol. 20, Issue 9, pp. 2287-91, 1992 (PubMed).

Bestor, Laudano, Mattaliano et al.: "Cloning and sequencing of a cDNA encoding DNA methyltransferase of mouse cells. The carboxyl-terminal domain of the mammalian enzymes is related to bacterial restriction methyltransferases." in: Journal of molecular biology, Vol. 203, Issue 4, pp. 971-83, 1989 (PubMed).

Xie, Wang, Okano et al.: "Cloning, expression and chromosome locations of the human DNMT3 gene family." in: Gene, Vol. 236, Issue 1, pp. 87-95, 1999 (PubMed).

Okano, Bell, Haber et al.: "DNA methyltransferases Dnmt3a and Dnmt3b are essential for de novo methylation and mammalian development." in: Cell, Vol. 99, Issue 3, pp. 247-57, 1999 (PubMed).

Reik, Kelsey, Walter: "Dissecting de novo methylation." in: Nature genetics, Vol. 23, Issue 4, pp. 380-2, 1999 (PubMed).

Robertson, Ait-Si-Ali, Yokochi et al.: "DNMT1 forms a complex with Rb, E2F1 and HDAC1 and represses transcription from E2F-responsive promoters." in: Nature genetics, Vol. 25, Issue 3, pp. 338-42, 2000 (PubMed).

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