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KCC2 antibody (AA 932-1043)

SLC12A5 Reactivity: Rat WB, IHC, IP, IF, ICC Host: Mouse Monoclonal S1-12 unconjugated
Catalog No. ABIN1027708
  • Target See all KCC2 (SLC12A5) Antibodies
    KCC2 (SLC12A5) (Solute Carrier Family 12 (Potassium-Chloride Transporter) Member 5 (SLC12A5))
    Binding Specificity
    • 12
    • 12
    • 8
    • 6
    • 5
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 932-1043
    Reactivity
    • 29
    • 25
    • 9
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Rat
    Host
    • 34
    • 14
    Mouse
    Clonality
    • 34
    • 14
    Monoclonal
    Conjugate
    • 24
    • 5
    • 4
    • 4
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    This KCC2 antibody is un-conjugated
    Application
    • 41
    • 32
    • 21
    • 14
    • 12
    • 11
    • 3
    • 2
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC)
    Specificity
    Detects ~140 kDa.
    Cross-Reactivity
    Human, Mouse, Rat
    Purification
    Protein G Purified
    Immunogen
    Fusion protein amino acids 932-1043 corresponding to rat KCC2
    Clone
    S1-12
    Isotype
    IgG2a
  • Application Notes
    • WB (1:1000)
    • IHC (1:300)
    • ICC/IF (1:100)
    • optimal dilutions for assays should be determined by the user.
    Comment

    1 μg/ml of ABIN1027708 was sufficient for detection of KCC2 in 10 μg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    -20°C
  • Target
    KCC2 (SLC12A5) (Solute Carrier Family 12 (Potassium-Chloride Transporter) Member 5 (SLC12A5))
    Alternative Name
    KCC2 (SLC12A5 Products)
    Synonyms
    Kcc2 antibody, KCC2 antibody, mKIAA1176 antibody, solute carrier family 12 member 5 antibody, solute carrier family 12, member 5 antibody, SLC12A5 antibody, Slc12a5 antibody
    Background
    KCC2 is a member of the cation-chloride cotransporter gene family (1). It acts as a K-Cl cotransporter. KCCs normally lower intracellular chloride concentrations below the electrochemical equilibrium potential and depending on the chemical concentration gradients of potassium and chloride, KCC2 can operate as a net efflux or influx pathway. It is proposed to act as the main chloride extruder to promote fast hyperpolarizing postsynaptic inhibition in the brain (2, 3). KCC2 is expressed at high levels in neurons throughout the nervous system and immunofluorescence shows that the protein is localized at inhibitory synapses of the spinal cord (4). Studies in mice have shown that KCC2 reduces GABA's inhibitory signaling, resulting in motor defects, epilepsy, and anxiety-like behavior.
    Gene ID
    171373
    NCBI Accession
    NP_599190
    UniProt
    Q63633
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