Insulin (INS) antibody

Antigen: Insulin (INS)

Synonyms: ILPR, IRDN, INS, MGC159719, ins1, xins, ins1-a, Insulin

Clonality: Monoclonal (E2-E3)

Host: Mouse

Reactivity: Human, Cow (Bovine), Pig (Porcine), Rabbit, Rat (Rattus)

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN102821

Additional Information

Alternative name: Insulin

Immunogen: Swine insulin.

Format: Ascites (prediluted)

Isotype: IgG1  (Matching secondary antibodies)

Clone: E2-E3

Specificity: Insulin is a hormone secreted by the beta cells of the islets of Langerhans in the pancreas. Insulin is a 51 amino acid polypeptide composed of A and B chains connected through the C-peptide. Insulin promotes glycogen storage, formation of triglycerides and synthesis of protein and nucleic acids. Cross reaction has been observed with insulin containing cells in fixed sections of pancreas from human, cow, pig, rabbit and rat.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Suitable for formalin/paraffin tissues. No pretreatment is required for paraffin sections. Positive Control: Pancreas. Cellular localization: Cytoplasmic

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as diluted ascites containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Diabetes, Hormones

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Images

Formalin fixed paraffin embedded human pancreas stained with Insulin antibody

Publications

Publications: Madsen: "Proinsulin-specific monoclonal antibodies. Immunocytochemical application as beta-cell markers and as probes for conversion." in: Diabetes, Vol. 36, Issue 10, pp. 1203-11, 1987 (PubMed).

Witt, Dietz, Ziegler et al.: "[Production and use of monoclonal glucagon and insulin antibodies--reduction of pancreatic insulin in rats by treatment with complete Freund's adjuvant]" in: Acta histochemica. Supplementband, Vol. 35, pp. 217-23, 1988 (PubMed).

Keilacker, Dietz, Witt et al.: "Kinetic properties of monoclonal insulin antibodies." in: Biomedica biochimica acta, Vol. 45, Issue 8, pp. 1093-102, 1986 (PubMed).