Insulin-Like Growth Factor 1 Receptor (IGF1R) antibody

Antigen: Insulin-Like Growth Factor 1 Receptor (IGF1R)

Synonyms: IGFR, CD221, IGFIR, JTK13, MGC18216, MGC142170, MGC142172, IGFIRC, IGF1R, IGF-1R, DKFZp469I0618, xIGFR, xigf1r, xIGF-1R, hyft, D930020L01, A330103N21Rik

Clonality: Monoclonal (24-31)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN102890

Additional Information

Alternative name: Insulin-Like Growth Factor-I Receptor (IGF-1 R)

Immunogen: BALB/C mice were immunized with IGF-1R/3t3 mouse fibroblasts transfected with human type I IGF-receptor cDNA.

Format: Purified

Isotype: IgG1  (Matching secondary antibodies)

Clone: 24-31

Specificity: This antibody stains with a 125 kD protein. Insulin-like Growth Factor-1 Receptor (IGF-1R) consists of alpha and beta-subunits, which are disulfide-linked in a beta-alpha-alpha-beta configuration in the mature receptor. The a-subunit is completely extracellular, while b-subunit spans the membrane and the intracellular portion has intrinsic tyrosine kinase activity. This antibody crossreacts with rabbit but does not react with rat.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 1 mM EDTA buffer, pH 8.0 prior to immunostaining. Positive Control: Breast carcinoma. Cellular localization: Cell membrane

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified ascites containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Growth Factors, Diabetes, Metabolism, Receptors

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Images

Formalin fixed paraffin embedded human placenta stained with IGF-1R antibody

Publications

Publications: Soos, Navue, Siddle: "Immunological studies of type I IGF receptors and insulin receptors: characterisation of hybrid and atypical receptor subtypes." in: Advances in experimental medicine and biology, Vol. 343, pp. 145-57, 1994 (PubMed).

Takahashi, Thomas, Williams: "Evidence for mutual interdependence of epithelium and stromal lymphoid cells in a subset of papillary carcinomas." in: British journal of cancer, Vol. 72, Issue 4, pp. 813-7, 1995 (PubMed).

Schumacher, Soos, Schlessinger et al.: "Signaling-competent receptor chimeras allow mapping of major insulin receptor binding domain determinants." in: The Journal of biological chemistry, Vol. 268, Issue 2, pp. 1087-94, 1993 (PubMed).