SARS-CoV Spike antibody (Intermediate Domain 3)

Catalog No. ABIN1031207

Product Details anti-SARS-CoV Spike Antibody

Target: SARS-CoV Spike (SARS-CoV S) (SARS-Coronavirus Spike Protein (SARS-CoV S))

Binding Specificity: Intermediate Domain 3

Reactivity: SARS Coronavirus (SARS-CoV)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SARS-CoV Spike antibody is un-conjugated

Application: ELISA

Predicted Reactivity: Predicted reactivity based on immunogen sequence: SARS-CoV2 Spike protein: (identity 40%, homology 56%)

Purification: Affinity chromatography purified via peptide column

Immunogen: Anti-SARS-CoV Spike antibody (3225) was raised against a peptide corresponding to 15 amino acids near the center of SARS-CoV Spike glycoprotein.
The immunogen is located within amino acids 650-700 of SARS-CoV Spike.

Isotype: IgG

Application Details

Application Notes: SARS-CoV Spike antibody can be used for the detection of SARS-CoV Spike protein in ELISA. It will detect 5 ng of free peptide at 1 μg/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: The antibody is supplied in PBS containing 0.02% sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Storage: 4 °C/-20 °C

Storage Comment: The antibody can be stored at 4 ˚ C for three months and -20 ˚ C, stable for up to one year.

Expiry Date: 12 months

Target Details for SARS-CoV Spike

Target: SARS-CoV Spike (SARS-CoV S) (SARS-Coronavirus Spike Protein (SARS-CoV S))

Alternative Name: SARS-CoV Spike (SARS-CoV S Products)

Target Type: Viral Protein

Background: Coronavirus disease 2019 (COVID-19), formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus. The disease is the cause of the 2019–20 coronavirus outbreak. The structure of 2019-nCoV consists of the following: a Spike protein (S), hemagglutinin-esterease dimer (HE), a membrane glycoprotein (M), an envelope protein (E) a nucleoclapid protein (N) and RNA. Coronavirus invades cells through Spike (S) glycoproteins, a class I fusion protein. It is the major viral surface protein that coronavirus uses to bind to the human cell surface receptor. It also mediates the fusion of host and viral cell membrane, allowing the virus to enter human cells and begin infection. The spike protein is the major target for neutralizing antibodies and vaccine development. The protein modeling suggests that there is strong interaction between Spike protein receptor-binding domain and its host receptor angiotensin-converting enzyme 2 (ACE2), which regulate both the cross-species and human-to-human transmissions of COVID-19. The recent study has shown that the SARS-CoV-2 spike protein binds ACE2 with higher affinity than SARS-CoV spike protein .

Gene ID: 1489668

NCBI Accession: P59594