Interleukin 6 (IL6) antibody

Antigen: Interleukin 6 (IL6)

Synonyms: HGF, HSF, BSF2, IL-6, IFNB2, Il-6, ILg6, Ifnb2, IL6, il-6

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Flow Cytometry (FACS), Immunocytochemistry (ICC), Neutralization (Neut), ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN103144

Additional Information

Alternative name: Interleukin 6 (IL-6)

Immunogen: This whole rabbit serum was prepared by repeated immunizations with recombinant human IL-6 produced in E.coli.

Format: Liquid (sterile filtered)

Specificity: This antiserum detects recombinant and native IL-6 present in body fluids and cell supernatants in various assays (i.e. IL-1 stimulated IL-6 production from fibroblasts). In Western blot analysis of natural cell products or human body fluids, multiple bands of IL-6 will appear due to the variable amount of glycosylation on the molecule. The antiserum is also useful for neutralization of human IL-6 activity in bioassays. For neutralization, incubate the sample with a 1:400 dilution of the antiserum for at least 4 hours before testing. A control of similarly diluted normal rabbit IgG (heat inactivated) is recommended. In neutralization experiments in vitro, this antibody does not result in enhanced activity of IL-6. However, because antibodies to IL-6 may act as a soluble receptor in vivo, some antibodies to IL-6 act as carriers and enhance IL-6 activity. Protein Sequence: Human Interleukin 6, 212 aa, predicted MW 23.7 kDa 1 mnsfstsafg pvafslglll vlpaafpapv ppgedskdva aphrqpltss eridkqiryi 61 ldgisalrke tcnksnmces skealaennl nlpkmaekdg cfqsgfneet clvkiitgll 121 efevyleylq nrfesseeqa ravqmstkvl iqflqkkakn ldaittpdpt tnaslltklq 181 aqnqwlqdmt thlilrsfke flqsslralr qm

Application Details

Application Notes: Recommended Dilutions: ELISA 1:1,000 - 1:5,000 Use as detection antibody WESTERN BLOT 1:500 - 1:2,000 Use 2 x 10 6 endotoxin-stimulated human PBMC IMMUNOHISTOCHEMISTRY 1:400 - 1:800 Use paraffin fixation or cryofixation IMMUNOPRECIPITATION 1:400 - 1:800 Pre-clearing with non-specific rabbit IgG RADIOIMMUNOASSAY 1:4,000 - 1:8,000 User Optimized NEUTRALIZATION 1:400. Pre-clearing with non-specific rabbit IgG. OTHER APPLICATIONS User Optimized. Note: This antiserum has been tested for use in neutralizations, ELISA, immunohistochemistry, radioimmunoassay, immunoprecipitation and immunoblotting. It recognizes IL-6. Reactivity in other immunoassays is unknown. For immunoblotting, use the supernatants or lysates of 2 x 10 6 endotoxin-stimulated human peripheral blood mononuclear cells (PBMC). PBMC are stimulated for 24 hours with 1% (v/v) human serum plus 10 ng/mL E.coli LPS. For immunohistochemistry, either paraffin fix or cryofix tissue. For immunoprecipitation, pre-clearing with a non-specific rabbit IgG is helpful to reduce background. For use in ELISA formats, this antibody is best used as the second antibody in combination with a monoclonal antibody as the capture antibody.

Concentration: 75 mg/ml (by Refractometry)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Storage: Store vial at -20° C prior to opening. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is six (6) months from date of opening.

Research Area: Cytokines, Receptors, Inflammation, Immunology, Cancer, Virology

Restrictions: For Research Use only

Images

Immunoblot using antibodies-online's anti- IL6 antibody. This blot shows detection of an IL-6-GST fusion protein (~300 ng, lane 1, green, 800 nm channel). Minimal reactivity is observed against GST (data not shown). Protein was resolved on a 4-20% Tris- Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking, the membrane was probed with the primary antibody diluted to 1:1,000. Incubation was overnight at 4

Publications

Publications: Kanbe, Inoue, Inoue et al.: "Inducement of mitogen-activated protein kinases in frozen shoulders." in: Journal of orthopaedic science : official journal of the Japanese Orthopaedic Association, Vol. 14, Issue 1, pp. 56-61, 2009 (PubMed).

Kanbe, Chen, Nakamura et al.: "Inhibition of MAP kinase in synovium by treatment with tocilizumab in rheumatoid arthritis." in: Clinical rheumatology, 2011 (PubMed).