Involucrin (IVL) antibody
| Antigen |
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| Synonyms |
1110019C06Rik, IVL, MGC143178 |
| Clonality |
Monoclonal (SY5) |
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Host
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Reactivity
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Human (31), Mouse (Murine) (16), Rat (Rattus) (15), Dog (Canine) (9), Pig (Porcine) (9), Cow (Bovine) (8), Monkey (3), Rabbit (3), Chicken (2), Sheep (Ovine) (2), Zebrafish (Brachydanio rerio) (1)
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Application
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 Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Immunofluorescence (IF) (17), Flow Cytometry (FACS) (16), Western Blotting (WB) (14), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (13), Immunoprecipitation (IP) (4), ELISA (3), Immunohistochemistry (Frozen Sections) (IHC (fro)) (3), Enzyme Immunoassay (EIA) (2), Immunohistochemistry (IHC) (2), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (IEM) (1)
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| Catalog no. |
ABIN103310 |
| Quantity |
1ml (100ug/ml approximately) (Variants) |
| Price |
372.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Alternative name
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Involucrin
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Immunogen
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BALB/C mice were injected with human keratinocytes involucrin.
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Format
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Supernatant
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Isotype
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IgG1 (Matching secondary antibodies)
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Clone
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SY5
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Specificity
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This antibody is specific to a 66 - 170 kD protein, which is identified as involucrin. This antibody stains the involucrin in a variety of sizes, 170 kD in MCF-7 cells, a doublet of ~115 kD and 150 kD in gorilla and monkey and a doublet of 105 kD in pig.
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Application Notes
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Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:75 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Positive Control: Skin. Cellular localization: Cytoplasmic Other Applications: Western Blotting (1:100-500).
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Concentration
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100ug/ml approximately
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Buffer
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This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative.
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Storage
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Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
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Research Area
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Extracellular Matrix
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Restrictions
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For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union
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Publications
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Hudson, Weiland, Dooley et al.: "Characterisation of eight monoclonal antibodies to involucrin." in: Hybridoma, Vol. 11, Issue 3, pp. 367-79, 1992 (PubMed).
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Alternatives for antigen "Involucrin (IVL)", type "Antibodies"
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Hosts
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Rabbit (27), Mouse (5)
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Reactivities
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Human (31), Mouse (Murine) (16), Rat (Rattus) (15), Dog (Canine) (9), Pig (Porcine) (9), Cow (Bovine) (8), Monkey (3), Rabbit (3), Chicken (2), Sheep (Ovine) (2), Zebrafish (Brachydanio rerio) (1)
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Applications
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Immunofluorescence (IF) (17), Flow Cytometry (FACS) (16), Western Blotting (WB) (14), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (13), Immunoprecipitation (IP) (4), ELISA (3), Immunohistochemistry (Frozen Sections) (IHC (fro)) (3), Enzyme Immunoassay (EIA) (2), Immunohistochemistry (IHC) (2), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (IEM) (1)
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Conjugates
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Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Epitopes
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AA 420-578 (5)
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