Nuclear Antigen (IPO-38) antibody

Antigen: Nuclear Antigen (IPO-38)

Clonality: Monoclonal (IP-O38)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN103314

Additional Information

Alternative name: IPO-38 (Proliferation Marker)

Immunogen: BALB/C mice were injected with spleen cells of a patient with hairy.

Format: Ig-fraction

Isotype: IgM kappa

Clone: IP-O38

Specificity: This antibody reacts with a 14-16 kD protein. IPO-38 antigen is present in the nuclei of proliferating cells such as Hodgkin

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:75 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the end user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Tonsil. Cellular localization: Nuclear

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications

Publications: Mathews, Bernstein, Franza et al.: "Identity of the proliferating cell nuclear antigen and cyclin." in: Nature, Vol. 309, Issue 5966, pp. 374-6, 1984 (PubMed).