Antigen Identified By Monoclonal antibody Ki-67 (MKI67) antibody

Antigen: Antigen Identified By Monoclonal Antibody Ki-67 (MKI67)

Synonyms: KIA, Ki67, Ki-67, D630048A14Rik, MKI67, kia, mki67, MGC132156

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN103390

Additional Information

Alternative name: Ki-67 Antigen

Immunogen: Synthetic peptide from 62 base pair region of the human Ki-67 antigen.

Format: Ig-fraction

Specificity: This antibody reacts with a nuclear antigen present in proliferating human cells. This antibody cross-reacts with a range of mammalian species. It can be used to evaluate the ki- 67 labeling index in various tumors.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Tonsil. Cellular localization: Nuclear

Concentration: 100ug/ml approximately

Buffer: Purified immunoglobulin fraction of rabbit antiserum against human Ki-67 antigen containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Proliferation Markers, Cell Cycle

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Images

Formalin fixed paraffin embedded human tonsil stained with Ki-67 antibody

Publications

Publications: Wintzer, Zipfel, Schulte-Moenting et al.: "Ki-67 immunostaining in human breast tumors and its relationship to prognosis." in: Cancer, Vol. 67, Issue 2, pp. 421-8, 1991 (PubMed).

Gerdes, Li, Schlueter et al.: "Immunobiochemical and molecular biologic characterization of the cell proliferation-associated nuclear antigen that is defined by monoclonal antibody Ki-67." in: The American journal of pathology, Vol. 138, Issue 4, pp. 867-73, 1991 (PubMed).

Brown, Gatter: "Monoclonal antibody Ki-67: its use in histopathology." in: Histopathology, Vol. 17, Issue 6, pp. 489-503, 1991 (PubMed).

Gerdes: "Ki-67 and other proliferation markers useful for immunohistological diagnostic and prognostic evaluations in human malignancies." in: Seminars in cancer biology, Vol. 1, Issue 3, pp. 199-206, 1991 (PubMed).

Song, Kojima, Hanamura et al.: "Expression of drebrin E in migrating neuroblasts in adult rat brain: coincidence between drebrin E disappearance from cell body and cessation of migration." in: Neuroscience, Vol. 152, Issue 3, pp. 670-82, 2008 (PubMed).

Franco, Chiesa, Beloti et al.: "Human osteoblastic cell response to a Ca- and P-enriched titanium surface obtained by anodization." in: Journal of biomedical materials research. Part A, Vol. 88, Issue 4, pp. 841-8, 2009 (PubMed).

de Oliveira, de Oliva, Maximiano et al.: "Effects of a mixture of growth factors and proteins on the development of the osteogenic phenotype in human alveolar bone cell cultures." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 56, Issue 7, pp. 629-38, 2008 (PubMed).