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Lactate Dehydrogenase (LDH) antibody

Details for Product No. ABIN103412
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Antigen
Synonyms CG10160, Dmel\\CG10160, IMP-L3, IMPL3, Imp-L3, Impl3, LDH, Ld, Ldh, dLdha, dmLDH
Reactivity
Rabbit
(5), (4), (1), (1)
Host
Goat
(6), (4), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(4)
Application
Western Blotting (WB), ELISA
(11), (11), (4), (4), (4), (3), (2)
Pubmed 5 references available
Quantity 2 mL
Options
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Catalog No. ABIN103412
361.90 $
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Immunogen This antibody was prepared from whole goat serum produced by repeated immunizations with a full length lactate dehydrogenase protein isolated from rabbit muscle.
Immunogentype:Native
Characteristics Concentration Definition: by Refractometry
Purification Antiserum
Alternative Name Lactate Dehydrogenase
Background Lactate dehydrogenase is also known as L-lactate dehydrogenase A chain, LDH-A, LDH muscle subunit and LDH-M. Two isozymes of LDH occur in mammals, LDH-M and LDH-H which come together to form a homotetramer of 36 kDa subunits. Every LDH molecule consists of four subunits, where each subunit is either H each M (based on their electrophoretic properties.) There are, therefore, five LDH isotypes: LDH-1 (4H) - in the heart, LDH-2 (3H1M) - in the reticuloendothelial system, LDH-3 (2H2M) - in the lungs, LDH-4 (1H3M) - in the kidneys and LDH-5 (4M) - in the liver and striated muscle.  Usually LDH-2 is the predominant form in the serum. An LDH-1 level higher than the LDH-2 level (a ''flipped pattern'') suggests myocardial infarction (damage to heart tissues releases heart LDH, which is rich in LDH-1, into the bloodstream). In general, LDH is often used as a marker of tissue breakdown.  LDH shows a cytoplasmic localization.
Synonyms: L-lactate dehydrogenase A chain LDH-A LDH muscle subunit LDH-M
Gene ID 100009107
NCBI Accession 126050
UniProt P13491
Research Area Enzymes, Metabolism
Application Notes This antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 36 kDa in size corresponding to LDH western blotting in the appropriate cell lysate or extract.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Restore with deionized water (or equivalent)
Concentration 82 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Supplier Images
anti-Lactate Dehydrogenase (LDH) antibody Western Blot of Goat Anti-Lactate Dehydrogenase antibody. Lane 1-4: HeLa cell extracts cytoplasmic fraction (CF). Lane 5-8: HeLa cell extracts nuclear fraction (NF). Load: 30 µg per lane. Primary antibody: LDH antibody at 1:400 for overnight at 4°C. Secondary antibody: IRDye800™ secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO/TBST overnight at 4°C. Predicted/Observed size: 36.6 kDa, 36 kDa for LDH. Other band(s): None.
General He, Borland, Zhu et al.: "Effect of ligand activation of peroxisome proliferator-activated receptor-beta/delta (PPARbeta/delta) in human lung cancer cell lines." in: Toxicology, Vol. 254, Issue 1-2, pp. 112-7, 2008 (PubMed).

Shiflett, Mauna, Chipman et al.: "Appetitive Pavlovian conditioned stimuli increase CREB phosphorylation in the nucleus accumbens." in: Neurobiology of learning and memory, Vol. 92, Issue 3, pp. 451-4, 2009 (PubMed).

Miyata, Hosoi, Mukai et al.: "In vitro import of peroxisome-targeting signal type 2 (PTS2) receptor Pex7p into peroxisomes." in: Biochimica et biophysica acta, Vol. 1793, Issue 5, pp. 860-70, 2009 (PubMed).

Liu, Zhang, Yin et al.: "alpha-Synuclein is differentially expressed in mitochondria from different rat brain regions and dose-dependently down-regulates complex I activity." in: Neuroscience letters, Vol. 454, Issue 3, pp. 187-92, 2009 (PubMed).

Foreman, Sharma, Amin et al.: "Ligand activation of peroxisome proliferator-activated receptor-beta/delta (PPARbeta/delta) inhibits cell growth in a mouse mammary gland cancer cell line." in: Cancer letters, 2009 (PubMed).

Validation Images
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