Laminin, alpha 1 (LAMA1) antibody

Antigen: Laminin, alpha 1 (LAMA1)

Synonyms: Lama, AA408497, LAMA, LAMA1

Clonality: Monoclonal (4C7)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN103446

Additional Information

Alternative name: Laminin

Immunogen: The A chain of human laminin.

Format: Supernatant

Isotype: IgG2a kappa  (Matching secondary antibodies)

Clone: 4C7

Specificity: This antibody reacts with the terminal globular domain of the A chain. It does not cross react with laminin from mouse or rat. The staining with this antibody is sensitive to fixation of paraffin tissues and use of proteolytic enzymatic treatment.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:5-1:20 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30-60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Proteolytic treatment of paraffin tissue sections is recommended. Positive Control: Skin. Cellular localization: Basement membrane

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as tissue culture supernatant containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Extracellular Matrix

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications

Publications: in:

Kleinman, Ogle, Cannon et al.: "Laminin receptors for neurite formation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 85, Issue 4, pp. 1282-6, 1988 (PubMed).