Major Vault Protein (MVP) antibody

Antigen: Major Vault Protein (MVP)

Synonyms: LRP, VAULT1, 2310009M24Rik, cb771, wu:fb52b05, wu:fc02g01, mvp, MGC68839, MVP, MGC127407, MGC145641, Tb05.45E22.810

Clonality: Monoclonal (1032)

Host: Mouse

Reactivity: Human, Rat (Rattus)

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)

Catalog no.: ABIN103631

Additional Information

Alternative name: LRP / MVP (Major Vault Protein)

Immunogen: BALB/C mice were injected with proteins precipitated with AER317 from the phosphocellulose column flow through of extract of human breast cancer MCF-7 cells.

Format: Ig-fraction

Isotype: IgG1  (Matching secondary antibodies)

Clone: 1032

Specificity: This antibody reacts with a 104 to 110 kD protein known Major Vault Protein (MVP). It is identical to lung-resistance related protein (LRP). Treatment of cells with estradiol increases the amount of MVP in nuclear extract. Antibodies to progesterone and glucocorticoid receptors are also able to co-immunoprecipitate the MVP. This antibody cross reacts with rat.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the end user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Breast. Cellular localization: Cytoplasmic Other Applications: Immunoprecipitation, Western Blotting

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Images

Formalin fixed paraffin embedded human breast carcinoma stained with LRP/MVP antibody

Publications

Publications: Abbondanza, Rossi, Roscigno et al.: "Interaction of vault particles with estrogen receptor in the MCF-7 breast cancer cell." in: The Journal of cell biology, Vol. 141, Issue 6, pp. 1301-10, 1998 (PubMed).