Luciferase antibody

Catalog No. ABIN103647

Product Details anti-Luciferase Antibody

Target: Luciferase

Reactivity: Vibrio fischeri

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Luciferase antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)

Characteristics:

Synonyms: rabbit anti-Luciferase Antibody, Alkanal monooxygenase alpha chain, Bacterial luciferase alpha chain, luxA, Alkanal monooxygenase beta chain, Bacterial luciferase beta chain, luxB

Background: Anti luciferase Antibody recognizes luciferase that is commonly used in biological research as a reporter to assess the transcriptional activity in cells that are transfected with a genetic construct containing the luciferase gene under the control of a promoter of interest. Luciferase can also be used to detect the level of cellular ATP in cell viability assays or for kinase activity assays. Additionally proluminescent molecules that are converted to luciferin upon activity of a particular enzyme can be used to detect enzyme activity in coupled or two-step luciferase assays. Such substrates have been used to detect caspase activity and cytochrome P450 activity, among others.

Gene Name: LUXB_VIBFI, luxA

Purification: This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Luciferase [Photobacterium fischerii].  Cross reactivity against Luciferase from other tissues and species may occur but have not been specifically determined.

Immunogen:

Immunogen: Luciferase [Photobacterium fischerii]

Immunogen Type: Native Protein

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:500 - 1:2,500

Application Note: Luciferase Antibody is suitable for western blotting, immunohistochemistry, immunoprecipitation, and ELISA. Researchers should determine optimal titers for applications that are not stated below.

Western Blot Dilution: 1:1,000 - 1:5,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:25,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 75 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride,  pH 7.2

Stabilizer: None

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 12 months

References for anti-Luciferase antibody (ABIN103647)

Kenny, Duval, Sammut, Steele, Pritchard, Atherton, Argent, Dimaline, Dockray, Varro: "Increased expression of the urokinase plasminogen activator system by Helicobacter pylori in gastric epithelial cells." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 295, Issue 3, pp. G431-41, (2008) (PubMed).

Target Details for Luciferase

Target: Luciferase

Abstract: Luciferase Products

Synonyms: hypothetical protein antibody, luciferase antibody, C25G6.3 antibody, Gbro_2186 antibody

Gene ID: 3280269

UniProt: P29238, P19907