Lysozyme (Muramidase) antibody

Antigen: Lysozyme (Muramidase)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN103750

Additional Information

Immunogen: Lysozyme isolated from urine of patients with monocytic leukemia.

Format: Ig-fraction, Purified

Specificity: This antibody reacts with lysozyme. It stains granulocytes, monocytes and macrophages in human tonsil, skin and colon. This antibody does not cross-react with any other cell types.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user. Suitable for formalin/paraffin tissues. Positive Control: Tonsil. Cellular localization: Cytoplasmic

Concentration: 100ug/ml approximately

Buffer: Purified immunoglobulin fraction of rabbit antiserum against human lysozyme containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Enzymes, Metabolism

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Images

Formalin fixed paraffin embedded human tonsil stained with Lysozyme antibody

Publications

Publications: in:

Krugliak, Meyer, Taylor: "The distribution of lysozyme, alpha-1-antitrypsin, and alpha-1-antichymotrypsin in normal hematopoietic cells and in myeloid leukemias: an immunoperoxidase study on cytocentrifuge preparations, smears, and paraffin sections." in: American journal of hematology, Vol. 21, Issue 1, pp. 99-109, 1986 (PubMed).