Macrophage (CD68) antibody

Antigen: Macrophage (CD68)

Clonality: Monoclonal (MAC387)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN103770

Additional Information

Alternative name: Macrophage

Immunogen: BALB/C mice were injected with affinity purified monocyte membrane preparation.

Format: Supernatant

Isotype: IgG1  (Matching secondary antibodies)

Clone: MAC387

Specificity: This antibody is specific to human leukocyte antigen L1, also known as calprotectin, calgranulin and cystic fibrosis antigen. This antibody reacts with neutrophils, monocytes, macrophages and squamous mucosal epithelia.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:75 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Proteolytic treatment of paraffin tissue sections is recommended. Positive Control: Tonsil. Cellular localization: Cytoplasmic

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Serum/Plasma Proteins

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications

Publications: Brandtzaeg, Dale, Gabrielsen: "The leucocyte protein L1 (calprotectin): usefulness as an immunohistochemical marker antigen and putative biological function." in: Histopathology, Vol. 21, Issue 2, pp. 191-6, 1992 (PubMed).

Loftus, Loh, Curran et al.: "Mac387: its non-specificity as a tumour marker or marker of histiocytes." in: Histopathology, Vol. 19, Issue 3, pp. 251-5, 1991 (PubMed).

Flavell, Jones, Wright: "Identification of tissue histiocytes on paraffin sections by a new monoclonal antibody." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 35, Issue 11, pp. 1217-26, 1987 (PubMed).