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Malondialdehyde / MDA antibody

Details for Product No. ABIN103910, Supplier: Log in to see
Immunohistochemistry (IHC)
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Immunogen Synthetic Malondialdehyde conjugated to bovine serum albumin (BSA)
Isotype IgG
Specificity Conjugated Malondialdehyde . Using a conjugate Malondialdehyde-Protein, antibody specificity was performed with an ELISA test by competition experiments with the following compounds: Compounds Cross-reactivity ratio (a) Malondialdehyde-BSA 1 4-hydroxynonenal-BSA 1/>100,000 Acrolein-BSA 1/>100,000 Formaldehyde-BSA 1/>100,000 Azelaic acid-BSA 1/>100,000 (a): Malondialdehyde-BSA concentration /conjugated close related structures concentration at half displacement. BSA = Bovine Serum Albumin.
Purification Antiserum previously preabsobed on protein carriers, and purified
Target Type Chemical
Application Notes Example of immunohistochemistry protocol Perfusion (Example for Adult male Sprague Dawley (weight around 0.5 kg)). 1-The animals can be deeply anaesthetized (for example with urethane-0.5-1.5g/kg, intraperitoneal). 2-Perfused via the ascending aorta with 50 ml of NaCl 9g/l (Heparinized) and pass trhough the system 800-1000 ml of cold 4% paraformaldehyde (Merck) in 0.1 M PB, pH 7.2-7.4, (ten minutes). 3-Dissect out the organs and place in a solution of 4% paraformaldehyde in 0.1M PB, pH 7.2, at 4ºC for twelve to sixteen hours. Immunohistochemistry 1-In order to avoid possible interference with endogenous peroxidase, free-floating sections will be treated with distilled water containing NH 3 (20%), H 2 O 2 (30%) and NaOH (1%) for 20 min (other method is using a solution with 33% of H 2 O 2 and 66% of methanol). 2-Then, wash the sections for 20 min in 0.15 M phosphate-buffered saline (PBS) (pH 7.2) 3-Pre-incubate for 30 min in PBS containing 2-10% (variable to adjust) of normal horse serum and 0.3% of Triton X-100 (mixed solution). 4-Incubate at room temperature (1h 30min) and overnight at 4º C in the same mixed solution containing the diluted anti- conjugated Malondialdehyde antibodies(1/1,000-1/5,000). 5-Then, the sections will be wash in PBS (30 min). 6-After that we will incubate for 60 min at room temperature with biotinylated anti-(species) immunogammaglobulin (Vector) diluted 1/200 in PBS. 7-Wash during 30 min with PBS. 8-Sections will be incubated for 1 h with a 1/100 diluted avidin-biotin-peroxidase complex (Vectastain) in the mixed solution. 9-After that we will wash the sections in PBS (30 min) 10-Wash with Tris-HCl buffer (pH 7.6)(10 min). 11-The tissue-bound peroxidase will be developed with H 2 O 2 using 3, 3' diaminobenzidine as chromogen. 12-Finally the sections will be rinsed with PBS and coverslipped with PBS/Glycerol (1/1). Recommended dilutions for Immunohistochemistry (1/1,000-1/5,000) Recommended dilutions for Western blot (1,000-2,000)
Restrictions For Research Use only
Format Lyophilized
Background publications Fan, Mitchell, Rhodes, Cai: "Alpha-Phenyl-n-tert-butyl-nitrone attenuates lipopolysaccharide-induced neuronal injury in the neonatal rat brain." in: Neuroscience, Vol. 151, Issue 3, pp. 737-44, 2008 (PubMed).

Karuppagounder, Xu, Shi, Chen, Pedrini, Pechman, Baker, Beal, Gandy, Gibson: "Thiamine deficiency induces oxidative stress and exacerbates the plaque pathology in Alzheimer's mouse model." in: Neurobiology of aging, 2008 (PubMed).

Mancuso, Davidzon, Kurlan, Tawil, Bonilla, Di Mauro, Powers: "Hereditary ferritinopathy: a novel mutation, its cellular pathology, and pathogenetic insights." in: Journal of neuropathology and experimental neurology, Vol. 64, Issue 4, pp. 280-94, 2005 (PubMed).

Zhou, Deo, Hosoya, Terasaki, Obrosova, Brosius, Kumagai: "Increased JNK phosphorylation and oxidative stress in response to increased glucose flux through increased GLUT1 expression in rat retinal endothelial cells." in: Investigative ophthalmology & visual science, Vol. 46, Issue 9, pp. 3403-10, 2005 (PubMed).