Melan A (MLANA) antibody

Antigen: Melan A (MLANA)

Synonyms: MART1, MART-1

Clonality: Monoclonal (A103)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN103965

Additional Information

Alternative name: MART-1/Melan-A

Immunogen: BALB/C mice were injected with recombinant MART-1 protein.

Format: Supernatant

Isotype: IgG1  (Matching secondary antibodies)

Clone: A103

Specificity: This antibody is specific to a 20 kD protein known as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. This antibody does not cross react with MAGE-1 or tyrosinase protein. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Melanoma. Cellular localization: Cytoplasmic Other Applications: Immunoprecipitation (1:50), Western Blotting (1:50 to 1:100)

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Cancer

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications

Publications: Chen, Stockert, Jungbluth et al.: "Serological analysis of Melan-A(MART-1), a melanocyte-specific protein homogeneously expressed in human melanomas." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 12, pp. 5915-9, 1996 (PubMed).