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Antibodies
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anti-Chymase 1, Mast Cell (CMA1) antibody
Chymase 1, Mast Cell (CMA1) antibody
| Antigen |
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| Synonyms |
CMA1, Cma1, CYH, MCT1, chymase, MGC119890, MGC119891, Mcp-5, Mcpt5, MMCP-5, MCP3P |
| Clonality |
Monoclonal (CC1) |
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Host
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Reactivity
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Application
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 Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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| Catalog no. |
ABIN103968 |
| Quantity |
1ml (100ug/ml approximately) (Variants) |
| Price |
451.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Alternative name
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Mast Cell Chymase
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Immunogen
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BALB/C mice were injected with a purified human skin Chymase.
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Format
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Ig-fraction
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Isotype
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IgG1 (Matching secondary antibodies)
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Clone
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CC1
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Specificity
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This antibody reacts with mast cells distributed in skin, synovium, lung and heart. Mast cells contain a number of preformed chemicals mediators such as histamine, Chymase, carboxypeptidase and proteolytic Tryptase. Human mast cell chymase is considered to be an important marker of mast cells as well as an important mediator of inflammation.
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Application Notes
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Titer/Working Dilution: This antibody may be diluted to a titer of 1:100-1:200 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the end user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Tonsil. Cellular localization: Cytoplasmic Other Applications: Western Blotting
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Concentration
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100ug/ml approximately
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Buffer
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This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative.
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Storage
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Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
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Research Area
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Enzymes
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Restrictions
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For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union
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Publications
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Buckley, McEuen, Walls: "The detection of mast cell subpopulations in formalin-fixed human tissues using a new monoclonal antibody specific for chymase." in: The Journal of pathology, Vol. 189, Issue 1, pp. 138-43, 2000 (PubMed).
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Alternatives for antigen "Chymase 1, Mast Cell (CMA1)", type "Antibodies"
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Hosts
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Rabbit (23), Mouse (3), Goat (2)
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Reactivities
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Human (28), Mouse (Murine) (19), Rat (Rattus) (19), Cow (Bovine) (2), Dog (Canine) (2), Horse (Equine) (2), Fish (1), Pig (Porcine) (1), Rabbit (1)
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Applications
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Flow Cytometry (FACS) (18), Immunofluorescence (IF) (18), Western Blotting (WB) (9), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (6), ELISA (5), Enzyme Immunoassay (EIA) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoprecipitation (IP) (2), Immunoelectron Microscopy (IEM) (1)
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Conjugates
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Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Epitopes
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C-Term (1), Center (1)
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