Mast Cell Tryptase antibody
| Antigen |
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| Synonyms |
MCP7L1, MMCP-7L, MGC95428, MCP7-LIKE, TMT, trpA, PRSS31 |
| Clonality |
Monoclonal (AA1) |
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Host
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Reactivity
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Application
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 Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
Flow Cytometry (FACS) (19), Immunofluorescence (IF) (18), Western Blotting (WB) (9), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (8), Immunohistochemistry (Frozen Sections) (IHC (fro)) (5), ELISA (4), Enzyme Immunoassay (EIA) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoprecipitation (IP) (2), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (IHC) (1)
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| Catalog no. |
ABIN103971 |
| Quantity |
1ml (100ug/ml approximately) (Variants) |
| Price |
451.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Immunogen
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BALB/C mice were injected with a purified human lung Tryptase.
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Format
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Ig-fraction
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Isotype
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IgG1 (Matching secondary antibodies)
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Clone
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AA1
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Specificity
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This antibody reacts with mast cells distributed in skin, synovium, lung and heart. This antibody does not bind with any other cell type. Mast cells contain a number of preformed chemicals mediators such as histamine, Chymase, carboxypeptidase and proteolytic Tryptase. Human mast cell tryptase is considered to be an important marker of mast cell activation as well as an important mediator of inflammation. This antibody cross reacts with monkey, dog and cat.
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Application Notes
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Titer/Working Dilution: This antibody may be diluted to a titer of 1:100-1:200 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the end user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Tonsil. Cellular localization: Cytoplasmic Other Applications: Western Blotting
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Concentration
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100ug/ml approximately
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Buffer
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This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative.
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Storage
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Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
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Research Area
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Enzymes
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Restrictions
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For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union
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Publications
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Walls, Jones, Williams et al.: "Immunohistochemical identification of mast cells in formaldehyde-fixed tissue using monoclonal antibodies specific for tryptase." in: The Journal of pathology, Vol. 162, Issue 2, pp. 119-26, 1991 (PubMed).
Walls, Bennett, McBride et al.: "Production and characterization of monoclonal antibodies specific for human mast cell tryptase." in: Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology, Vol. 20, Issue 5, pp. 581-9, 1991 (PubMed).
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Alternatives for antigen "Mast Cell Tryptase", type "Antibodies"
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Hosts
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Rabbit (20), Mouse (7)
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Reactivities
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Human (27), Mouse (Murine) (20), Rat (Rattus) (20), Cow (Bovine) (16), Horse (Equine) (16), Pig (Porcine) (16), Rabbit (15), Dog (Canine) (4), Monkey (3), Cat (Feline) (2)
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Applications
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Flow Cytometry (FACS) (19), Immunofluorescence (IF) (18), Western Blotting (WB) (9), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (8), Immunohistochemistry (Frozen Sections) (IHC (fro)) (5), ELISA (4), Enzyme Immunoassay (EIA) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoprecipitation (IP) (2), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (IHC) (1)
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Conjugates
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Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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