Minichromosome Maintenance Complex Component 5 (MCM5) antibody

Antigen: Minichromosome Maintenance Complex Component 5 (MCM5)

Synonyms: CDC46, MGC5315, P1-CDC46, Cdc46, Mcmd5, mCD46, mCDC46, AA617332, AI324988, AL033333, mcm5-b, MGC68977, mcm5, MGC97563, 23.m06024

Clonality: Monoclonal (CRCT5-1)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN104090

Additional Information

Alternative name: MCM5

Immunogen: BALB/C mice were immunized with a Bacterially expressed human MCM5 protein.

Format: Purified

Isotype: IgG2b  (Matching secondary antibodies)

Clone: CRCT5-1

Specificity: This antibody with a protein of 90 kD. Mini Chromosome Maintainance Protein-5 (MCM5) is involved in the control of DNA replication. MCM proteins form a complex, which exhibits ATPase and helicase activities. In G0 phase levels of MCM2 and MCM5 are lower than that of MCM7 and 3 but the level increases as the cell enters G1/s phase of the cell cycle.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Cervical carcinoma. Cellular localization: Nuclear

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified ascites containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications

Publications: Todorov, Attaran, Kearsey: "BM28, a human member of the MCM2-3-5 family, is displaced from chromatin during DNA replication." in: The Journal of cell biology, Vol. 129, Issue 6, pp. 1433-45, 1995 (PubMed).

Paul, Hu, Musahl et al.: "Coding sequence and chromosome mapping of the human gene (CDC46) for replication protein hCdc46/Mcm5." in: Cytogenetics and cell genetics, Vol. 73, Issue 4, pp. 317-21, 1996 (PubMed).

Ishimi, Ichinose, Omori et al.: "Binding of human minichromosome maintenance proteins with histone H3." in: The Journal of biological chemistry, Vol. 271, Issue 39, pp. 24115-22, 1996 (PubMed).

Ishimi, Komamura, You et al.: "Biochemical function of mouse minichromosome maintenance 2 protein." in: The Journal of biological chemistry, Vol. 273, Issue 14, pp. 8369-75, 1998 (PubMed).