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Melanoma Associated Antigen (MAGE) antibody

Antigen

Melanoma Associated Antigen (MAGE)
Clonality Monoclonal (HMB45)
Host
Alternatives

Mouse
Reactivity
Alternatives

Human
Application
Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
2 references available
Catalog no. ABIN104125
Quantity 6ml  (1 to 4 ug/ml)  (Variants)
Price 209.00 $   Plus shipping costs $45.00
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Additional Information
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Alternative name Melanoma Associated Antigen
Immunogen BALB/C mice were injected with extract of pigmented melanoma metastases from lymph nodes.
Format Supernatant (prediluted)
Isotype IgG1 kappa
Clone HMB45
Specificity This antibody reacts with a neuraminidase sensitive oligosaccharide side chain of a glycoconjugate present in immature melanosomes. It reacts with junctional and blue nevus cells. Non-melanocytic cells are negative.

Application Details
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Application Notes Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Melanoma. Cellular localization: Cytoplasmic
Concentration 1 to 4 ug/ml
Purification Supernatant (prediluted)
Buffer This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required.
Storage Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
Research Area Cancer
Restrictions For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications
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Kapur, Bigler, Skelly et al.: "Anti-melanoma monoclonal antibody HMB45 identifies an oncofetal glycoconjugate associated with immature melanosomes." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 40, Issue 2, pp. 207-12, 1992 (PubMed).

Gown, Vogel, Hoak et al.: "Monoclonal antibodies specific for melanocytic tumors distinguish subpopulations of melanocytes." in: The American journal of pathology, Vol. 123, Issue 2, pp. 195-203, 1986 (PubMed).